Mary A Oboh1, Tolla Ndiaye2, Khadim Diongue3, Yaye D Ndiaye3, Mouhamad Sy3, Awa B Deme3, Mamadou A Diallo3, Mamadou S Yade3, Sarah K Volkman4, Aida S Badiane3, Alfred Amambua-Ngwa5, Daouda Ndiaye3,4. 1. Medical Research Council Unit, the Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia. aigbi4god@gmail.com. 2. Laboratory of Parasitology and Mycology, Aristide Le Dantec University Hospital, Cheikh Anta Diop University of Dakar, PO Box 5005, Dakar, Senegal. ndiayetola@gmail.com. 3. Laboratory of Parasitology and Mycology, Aristide Le Dantec University Hospital, Cheikh Anta Diop University of Dakar, PO Box 5005, Dakar, Senegal. 4. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA. 5. Medical Research Council Unit, the Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia.
Abstract
BACKGROUND: Characterizing the genetic diversity of malaria parasite populations in different endemic settings (from low to high) could be helpful in determining the effectiveness of malaria interventions. This study compared Plasmodium falciparum parasite population diversity from two sites with low (pre-elimination) and high transmission in Senegal and Nigeria, respectively. METHODS: Parasite genomic DNA was extracted from 187 dried blood spot collected from confirmed uncomplicated P. falciparum malaria infected patients in Senegal (94) and Nigeria (93). Allelic polymorphism at merozoite surface protein 1 (msp1) and merozoite surface protein- 2 (msp2) genes were assessed by nested PCR. RESULTS: The most frequent msp1 and msp2 allelic families are the K1 and IC3D7 allelotypes in both Senegal and Nigeria. Multiplicity of infection (MOI) of greater that 1 and thus complex infections was common in both study sites in Senegal (Thies:1.51/2.53; Kedougou:2.2/2.0 for msp1/2) than in Nigeria (Gbagada: 1.39/1.96; Oredo: 1.35/1.75]). The heterozygosity of msp1 gene was higher in P. falciparum isolates from Senegal (Thies: 0.62; Kedougou: 0.53) than isolates from Nigeria (Gbagada: 0.55; Oredo: 0.50). In Senegal, K1 alleles was associated with heavy than with moderate parasite density. Meanwhile, equal proportions of K1 were observed in both heavy and moderate infection types in Nigeria. The IC3D7 subtype allele of the msp2 family was the most frequent in heavily parasitaemic individuals from both countries than in the moderately infected participants. CONCLUSION: The unexpectedly low genetic diversity of infections high endemic Nigerian setting compared to the low endemic settings in Senegal is suggestive of possible epidemic outbreak in Nigeria.
BACKGROUND: Characterizing the genetic diversity of malaria parasite populations in different endemic settings (from low to high) could be helpful in determining the effectiveness of malaria interventions. This study compared Plasmodium falciparum parasite population diversity from two sites with low (pre-elimination) and high transmission in Senegal and Nigeria, respectively. METHODS: Parasite genomic DNA was extracted from 187 dried blood spot collected from confirmed uncomplicated P. falciparum malaria infectedpatients in Senegal (94) and Nigeria (93). Allelic polymorphism at merozoite surface protein 1 (msp1) and merozoite surface protein- 2 (msp2) genes were assessed by nested PCR. RESULTS: The most frequent msp1 and msp2 allelic families are the K1 and IC3D7 allelotypes in both Senegal and Nigeria. Multiplicity of infection (MOI) of greater that 1 and thus complex infections was common in both study sites in Senegal (Thies:1.51/2.53; Kedougou:2.2/2.0 for msp1/2) than in Nigeria (Gbagada: 1.39/1.96; Oredo: 1.35/1.75]). The heterozygosity of msp1 gene was higher in P. falciparum isolates from Senegal (Thies: 0.62; Kedougou: 0.53) than isolates from Nigeria (Gbagada: 0.55; Oredo: 0.50). In Senegal, K1 alleles was associated with heavy than with moderate parasite density. Meanwhile, equal proportions of K1 were observed in both heavy and moderate infection types in Nigeria. The IC3D7 subtype allele of the msp2 family was the most frequent in heavily parasitaemic individuals from both countries than in the moderately infectedparticipants. CONCLUSION: The unexpectedly low genetic diversity of infections high endemic Nigerian setting compared to the low endemic settings in Senegal is suggestive of possible epidemic outbreak in Nigeria.
Authors: Segun Isaac Oyedeji; Henrietta Oluwatoyin Awobode; Chiaka Anumudu; Jürgen Kun Journal: Asian Pac J Trop Med Date: 2013-08 Impact factor: 1.226
Authors: Babatunde A Odugbemi; Kikelomo O Wright; Adebayo T Onajole; Yetunde A Kuyinu; Olayinka O Goodman; Tinuola O Odugbemi; Olumuyiwa O Odusanya Journal: Malar J Date: 2016-09-07 Impact factor: 2.979
Authors: Akua Agyeman-Budu; Charles Brown; George Adjei; Mohammed Adams; David Dosoo; Dominic Dery; Michael Wilson; Kwaku P Asante; Brian Greenwood; Seth Owusu-Agyei Journal: Malar J Date: 2013-01-17 Impact factor: 2.979
Authors: James G Beeson; Damien R Drew; Michelle J Boyle; Gaoqian Feng; Freya J I Fowkes; Jack S Richards Journal: FEMS Microbiol Rev Date: 2016-01-31 Impact factor: 16.408