Literature DB >> 3343316

Purification, partial characterization, and seroreactivity of a genuswide 60-kilodalton Legionella protein antigen.

C P Pau1, B B Plikaytis, G M Carlone, I M Warner.   

Abstract

A genuswide protein antigen extracted from Legionella pneumophila serogroup 1 (strain Philadelphia 1) cells was enriched by differential pelleting and ammonium sulfate precipitation and subsequently purified with a combination of high-performance size-exclusion and ion-exchange chromatography. The protein has an apparent molecular weight of 650,000 before and 63,000 after urea (5 M) treatment, as determined by size-exclusion chromatography. These proteins resolved to a single band of 60,000 after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The urea-treated protein had an isoelectric point of 5.8. This purified 60-kilodalton protein reacted with a convalescent-phase serum sample from a patient with legionellosis and rabbit immune sera prepared against each of 23 Legionella species. The 60-kilodalton protein may be useful in developing diagnostic tests for legionellosis.

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Year:  1988        PMID： 3343316      PMCID： PMC266188          DOI： 10.1128/jcm.26.1.67-71.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.

Authors:  B B Plikaytis; G M Carlone; C P Pau; H W Wilkinson
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

2.  Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base-pair number after gel electrophoresis.

Authors:  B D Plikaytis; G M Carlone; P Edmonds; L W Mayer
Journal:  Anal Biochem       Date:  1986-02-01       Impact factor: 3.365

3.  Numerical analysis of normalized whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

Authors:  B D Plikaytis; G M Carlone; B B Plikaytis
Journal:  J Gen Microbiol       Date:  1986-09

4.  Calibration of prestained protein molecular weight standards for use in the "Western" or enzyme-linked immunoelectrotransfer blot techniques.

Authors:  V C Tsang; K Hancock; A R Simons
Journal:  Anal Biochem       Date:  1984-12       Impact factor: 3.365

5.  Differential determination of the 3-Deoxy-D-mannooctulosonic acid residues in lipopolysaccharides of Salmonella minnesota rough mutants.

Authors:  H Brade; C Galanos; O Lüderitz
Journal:  Eur J Biochem       Date:  1983-03-01

6.  Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness.

Authors:  A L Reingold; B M Thomason; B J Brake; L Thacker; H W Wilkinson; J N Kuritsky
Journal:  J Infect Dis       Date:  1984-05       Impact factor: 5.226

7.  Immunologic response of patients with legionellosis against major protein-containing antigens of Legionella pneumophila serogroup 1 as shown by immunoblot analysis.

Authors:  J S Sampson; B B Plikaytis; H W Wilkinson
Journal:  J Clin Microbiol       Date:  1986-01       Impact factor: 5.948

8.  Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease.

Authors:  J E McDade; C C Shepard; D W Fraser; T R Tsai; M A Redus; W R Dowdle
Journal:  N Engl J Med       Date:  1977-12-01       Impact factor: 91.245

9.  Immune serum to protein molecular weight standards for calibrating Western blots.

Authors:  G M Carlone; B B Plikaytis; R J Arko
Journal:  Anal Biochem       Date:  1986-05-15       Impact factor: 3.365

10.  Gas-liquid chromatography as an analytical tool in microbiology.

Authors:  C W Moss
Journal:  J Chromatogr       Date:  1981-01-09
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  8 in total

1.  Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila.

Authors:  J S Sampson; B B Plikaytis; C H Aloisio; G M Carlone; C P Pau; A R Stinson
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

2.  Immunolocalization of Hsp60 in Legionella pneumophila.

Authors:  R A Garduño; G Faulkner; M A Trevors; N Vats; P S Hoffman
Journal:  J Bacteriol       Date:  1998-02       Impact factor: 3.490

3.  Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.

Authors:  I Steinmetz; C Rheinheimer; I Hübner; D Bitter-Suermann
Journal:  J Clin Microbiol       Date:  1991-02       Impact factor: 5.948

4.  Newly characterized species-specific immunogenic Chlamydophila pneumoniae peptide reactive with murine monoclonal and human serum antibodies.

Authors:  Eric L Marston; Andrea V James; J Todd Parker; John C Hart; Teresa M Brown; Trudy O Messmer; Danny L Jue; Carolyn M Black; George M Carlone; Edwin W Ades; Jacquelyn Sampson
Journal:  Clin Diagn Lab Immunol       Date:  2002-03

5.  Immunoreactivity of five monoclonal antibodies against the 37-kilodalton common cell wall protein (PsaA) of Streptococcus pneumoniae.

Authors:  J Crook; J A Tharpe; S E Johnson; D B Williams; A R Stinson; R R Facklam; E W Ades; G M Carlone; J S Sampson
Journal:  Clin Diagn Lab Immunol       Date:  1998-03

6.  Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, induces protective immunity in a guinea pig model of Legionnaires' disease.

Authors:  S J Blander; M A Horwitz
Journal:  J Clin Invest       Date:  1993-02       Impact factor: 14.808

7.  Surface-associated hsp60 chaperonin of Legionella pneumophila mediates invasion in a HeLa cell model.

Authors:  R A Garduño; E Garduño; P S Hoffman
Journal:  Infect Immun       Date:  1998-10       Impact factor: 3.441

8.  The Legionella pneumophila Chaperonin - An Unusual Multifunctional Protein in Unusual Locations.

Authors:  Rafael A Garduño; Audrey Chong; Gheyath K Nasrallah; David S Allan
Journal:  Front Microbiol       Date:  2011-06-10       Impact factor: 5.640
  8 in total

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