Protective role of rutin against combined exposure to lipopolysaccharide and D-galactosamine-induced dysfunctions in liver, kidney, and brain: Hematological, biochemical, and histological evidences.

Protective efficacy of rutin over liver, kidney, and brain dysfunctions was evaluated in this investigation. Rutin (5, 10, and 20 mg/kg) was administered continuously for 6 days followed by single dose of D-galactosamine (300 mg/kg I.P.) and lipopolysaccharide (50 µg/kg I.P.) on the 6th day. Hematological, serological, biochemical, and histological aspects were considered for this study. One-way ANOVA (p ≤ .05) followed by Tukey's HSD post hoc test determined the statistical significance. Serum AST, ALT, ALP, urea, uric acid, and creatinine were increased significantly, whereas albumin and glucose were significantly decreased after combined exposure to LPS and D-GalN. Glutathione level and activity of SOD and catalase were decreased, whereas lipid peroxidation, triglycerides, and cholesterol were increased in tissue samples due to LPS- and D-GalN-induced toxicity. Prophylactic treatment of rutin maintained studied variables toward control claiming the protective role of rutin. PRACTICAL APPLICATION: Rutin is plenteous in a variety of commonly ingested foods such as onion, wine, grape, citrus fruits, tea, and buckwheat. Rutin supplement is recommended for the treatment of various diseases such as varicose veins, internal bleeding, or hemorrhoids. Rutin is better than well-known antithrombic agent, Juniferdin, or Bacitracin. In the present study, rutin showed protective effects against LPS- and D-GalN-induced multiorgan dysfunctions due to its anti-inflammatory and antioxidant properties. Therefore, rutin may be developed and practiced as a food supplement to cope with acute organ dysfunctions caused by inflammatory and oxidative damage.