| Literature DB >> 33431892 |
Liu Yang1, Haiyan Yang2, Yunxiang Chu3, Yunhao Song1, Lidan Ding1, Bingtao Zhu1, Wanli Zhai1, Xuning Wang4, Yanshen Kuang4, Fangli Ren1, Baoqing Jia4, Wei Wu2, Xiongjun Ye5, Yinyin Wang6, Zhijie Chang7.
Abstract
Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of the intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting protein, is required for the maintenance of murine ISCs. CREPT is preferably expressed in the crypts but not in the villi. Deletion of CREPT in the intestinal epithelium of mice (Vil-CREPTKO) results in lower body weight and slow migration of epithelial cells in the intestine. Vil-CREPTKO intestine fails to regenerate after X-ray irradiation and dextran sulfate sodium (DSS) treatment. Accordingly, the deletion of CREPT decreases the expression of genes related to the proliferation and differentiation of ISCs and reduces Lgr5+ cell numbers at homeostasis. We identify that CREPT deficiency downregulates Wnt signaling by impairing β-catenin accumulation in the nucleus of the crypt cells during regeneration. Our study provides a previously undefined regulator of ISCs.Entities:
Year: 2021 PMID： 33431892 DOI： 10.1038/s41467-020-20636-9
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919