Xilin Liu1, Xianji Song2, Hong Li3. 1. Department of Hand Surgery, China Japan Union Hospital of Jilin University, Changchun, 130033, Jilin, China. 2. Orthopaedic Surgery, China Japan Union Hospital of Jilin University, Changchun, 130033, Jilin, China. 3. Emergency Medical of China Japan Union Hospital of Jilin University, No. 126 Xian Tai Street, Changchun, 130033, Jilin, China. hongli2018@jlu.edu.cn.
Abstract
BACKGROUND: Ectopic expression of transcription elongation factor A (SII)-like 7 (TCEAL7) has been observed in several kinds of cancers, but its role in melanoma is still unclear. This study was carried out to investigate TCEAL7 role in melanoma progression, and uncover the underlying mechanisms. METHODS: TCEAL7 expression levels in melanoma tissues and cells were determined by using real-time quantitative PCR (RT-PCR) and western blotting. CCK-8, transwell chambers, flow cytometry, starch assay and tumorigenesis assay were applied to detect cell growth, invasion, apoptosis, migration and tumorigenesis, respectively. RESULTS: A low expression level of TCEAL7 was observed in melanoma tissues and cells, which was associated with malignant clinical process and poor prognosis. TCEAL7 negatively modulated AKT1, AKT2, c-Myc, N-cadherin and PCNA expression and inhibited cancer progression via decreasing AKT1 and c-Myc levels. In addition, TCEAL7 was negatively modulated by miR-758-3p which promoted melanoma progression. Moreover, overexpression of TCEAL7 abolished miR-758-3p role in promoting melanoma progression. CONCLUSION: This study demonstrated that TCEAL7, regulated by miR-758-3p inhibited melanoma progression through decreasing the expression levels of c-Myc and AKT1.
BACKGROUND: Ectopic expression of transcription elongation factor A (SII)-like 7 (TCEAL7) has been observed in several kinds of cancers, but its role in melanoma is still unclear. This study was carried out to investigate TCEAL7 role in melanoma progression, and uncover the underlying mechanisms. METHODS:TCEAL7 expression levels in melanoma tissues and cells were determined by using real-time quantitative PCR (RT-PCR) and western blotting. CCK-8, transwell chambers, flow cytometry, starch assay and tumorigenesis assay were applied to detect cell growth, invasion, apoptosis, migration and tumorigenesis, respectively. RESULTS: A low expression level of TCEAL7 was observed in melanoma tissues and cells, which was associated with malignant clinical process and poor prognosis. TCEAL7 negatively modulated AKT1, AKT2, c-Myc, N-cadherin and PCNA expression and inhibited cancer progression via decreasing AKT1 and c-Myc levels. In addition, TCEAL7 was negatively modulated by miR-758-3p which promoted melanoma progression. Moreover, overexpression of TCEAL7 abolished miR-758-3p role in promoting melanoma progression. CONCLUSION: This study demonstrated that TCEAL7, regulated by miR-758-3p inhibited melanoma progression through decreasing the expression levels of c-Myc and AKT1.
Authors: J Chien; K Narita; R Rattan; S Giri; R Shridhar; J Staub; D Beleford; J Lai; L R Roberts; J Molina; S H Kaufmann; G C Prendergast; V Shridhar Journal: Oncogene Date: 2008-09-22 Impact factor: 9.867
Authors: Jeremy Chien; Julie Staub; Rajeswari Avula; Heyu Zhang; Wanguo Liu; Lynn C Hartmann; Scott H Kaufmann; David I Smith; Viji Shridhar Journal: Oncogene Date: 2005-07-28 Impact factor: 9.867