Ling He1,2, Xiaofan Jia1,3, Yong Gu1, Dongmei Miao1, Kathleen Waugh1, Cristy Geno1, Edwin Liu4, Marian Rewers1, Liping Yu1. 1. Barbara Davis Center for Diabetes, University of Colorado School of Medicine, Aurora, CO, USA. 2. Department of Endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China. 3. Department of Endocrinology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, China. 4. University of Colorado Children's Hospital, Aurora, CO, USA.
Abstract
BACKGROUND: Autoimmunity Screening for Kids (ASK) study was launched to screen general population children for type 1 diabetes (T1D) and celiac disease (CD). METHODS: A total of 23,319 children from general population were screened. A high throughput multiplex electrochemiluminescence (ECL) assay to screen multiautoantibodies in a single well was applied, parallel with a standard radiobinding assay (RBA). All children with any positive autoantibodies in screening were revisited within one month for confirmation and followed every 6 months. RESULTS: Among 23,319 children, 2.6% (606/23,319) of children were tested positive for TGA. Multiplex ECL assay detected more TGA (584/23,319) in the initial screening than RBA (490/23,319, p = 0.004) and was able to detect TGA earlier than RBA in a subset of children by 0.8 to 34.8 months. Prevalence of TGA by either ECL or RBA in children with islet autoantibodies was found significantly higher than overall prevalence in general population screened. CONCLUSIONS: A multiplex ECL assay was more sensitive than standard RBA by identifying more TGA positivity and detecting TGA earlier in general population screening. It also provides a high efficient tool with its unique advantage of multiplexing measurements to screen for multiple autoimmune diseases simultaneously in general population.
BACKGROUND: Autoimmunity Screening for Kids (ASK) study was launched to screen general population children for type 1 diabetes (T1D) and celiac disease (CD). METHODS: A total of 23,319 children from general population were screened. A high throughput multiplex electrochemiluminescence (ECL) assay to screen multiautoantibodies in a single well was applied, parallel with a standard radiobinding assay (RBA). All children with any positive autoantibodies in screening were revisited within one month for confirmation and followed every 6 months. RESULTS: Among 23,319 children, 2.6% (606/23,319) of children were tested positive for TGA. Multiplex ECL assay detected more TGA (584/23,319) in the initial screening than RBA (490/23,319, p = 0.004) and was able to detect TGA earlier than RBA in a subset of children by 0.8 to 34.8 months. Prevalence of TGA by either ECL or RBA in children with islet autoantibodies was found significantly higher than overall prevalence in general population screened. CONCLUSIONS: A multiplex ECL assay was more sensitive than standard RBA by identifying more TGA positivity and detecting TGA earlier in general population screening. It also provides a high efficient tool with its unique advantage of multiplexing measurements to screen for multiple autoimmune diseases simultaneously in general population.
Authors: Prashant Singh; Ananya Arora; Tor A Strand; Daniel A Leffler; Carlo Catassi; Peter H Green; Ciaran P Kelly; Vineet Ahuja; Govind K Makharia Journal: Clin Gastroenterol Hepatol Date: 2018-03-16 Impact factor: 11.382
Authors: Marcella Li; Liping Yu; Claudio Tiberti; Margherita Bonamico; Iman Taki; Dongmei Miao; Joseph A Murray; Marian J Rewers; Edward J Hoffenberg; Daniel Agardh; Patricia Mueller; Martin Stern; Ezio Bonifacio; Edwin Liu Journal: Am J Gastroenterol Date: 2009-01 Impact factor: 10.864
Authors: Edwin Liu; Fei Bao; Katherine Barriga; Dongmei Miao; Liping Yu; Henry A Erlich; Joel E Haas; George S Eisenbarth; Marian J Rewers; Edward J Hoffenberg Journal: Clin Gastroenterol Hepatol Date: 2003-09 Impact factor: 11.382
Authors: Marisa G Stahl; Cristy Geno Rasmussen; Fran Dong; Kathleen Waugh; Jill M Norris; Judith Baxter; Liping Yu; Andrea K Steck; Brigitte I Frohnert; Edwin Liu; Marian J Rewers Journal: Am J Gastroenterol Date: 2021-01-01 Impact factor: 12.045