Dina Salem Fotoh1, Rasha Ibrahim Noreldin2, Mohamed Soliman Rizk3, Maha Mohamed Elsabaawy4, Heba Ahmed Esaily1. 1. Physical Medicine, Rheumatology and Rehabilitation Department, Faculty of Medicine, Menoufia University, Egypt. 2. Clinical Pathology Department, Faculty of Medicine, Menoufia University, Egypt. 3. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt. 4. Hepatology and Gastroenterology Department, National Liver Institute, Menoufia University, Egypt.
Abstract
BACKGROUND: Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). AIM: Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. METHODS: A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. RESULTS: Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls (7.0 ± 3.4 vs. 4.1 ± 2.1 vs. 2.6 ± 0.6, p < 0.001, respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2.2 ± 1.1 vs. 4.1 ± 2.3 vs. 7.1 ± 4.5, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. CONCLUSIONS: Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications.
BACKGROUND: Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). AIM: Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. METHODS: A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. RESULTS: Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls (7.0 ± 3.4 vs. 4.1 ± 2.1 vs. 2.6 ± 0.6, p < 0.001, respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2.2 ± 1.1 vs. 4.1 ± 2.3 vs. 7.1 ± 4.5, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. CONCLUSIONS: Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications.
Authors: Sang-Woo Kim; Kumaraguruparan Ramasamy; Hakim Bouamar; An-Ping Lin; Daifeng Jiang; Ricardo C T Aguiar Journal: Proc Natl Acad Sci U S A Date: 2012-05-01 Impact factor: 11.205
Authors: Klára Prajzlerová; Kristýna Grobelná; Markéta Hušáková; Šárka Forejtová; Astrid Jüngel; Steffen Gay; Jiří Vencovský; Karel Pavelka; Ladislav Šenolt; Mária Filková Journal: PLoS One Date: 2017-09-22 Impact factor: 3.240