Literature DB >> 33423236

Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function, and Cell Fate Along Neural Deregulation.

Mariana Dutra Brito1, Luiz Felipe Souza E Silva1, Amanda Siena1, Miruna Chipara2, Sovan Sarkar2, Tatiana Rosado Rosenstock3,4.   

Abstract

Depletion of oxygen (O2) levels and reduction in the ATP synthesis (or even its complete blockage) are important characteristics of mitochondrial dysfunction; features that are often correlated with neurodegeneration. The measurement of oxygen consumption rate (OCR) is thus essential to evaluate cellular metabolism, survival, and neuroprotective strategies. In the present chapter, we describe the oxygen consumption assay using a Clark-type oxygen electrode in different types of samples named cells suspension (from primary and established cell culture), brain slices (ex vivo), and fresh brain tissues. In addition, we demonstrate herein how the program Oxygraph can be used in order to analyze the data and different approaches to normalize it.

Entities:  

Keywords:  Brain slice; Cell suspension; Cellular function; Metabolism; Mitochondria; Neural deregulation

Mesh：

Substances：

Year:  2021        PMID： 33423236     DOI： 10.1007/978-1-0716-1091-6_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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