| Literature DB >> 33423233 |
Taisa Magnani Dinamarco1, Paula Fagundes de Gouvêa2, Emerson de Souza Santos3, Lucas Oliveira Sousa3.
Abstract
The biological functions of a cell may change in response to exposure to toxic agents. Toxicogenomics employs the recent developments in genomics, transcriptomics, and proteomics to study how a chemical impacts gene/protein expression and cell functions. We describe a method for transcriptomic analysis by RNA sequencing based on Illumina HiSeq, NextSeq, or NovaSeq Systems followed by real-time qPCR validation. We also depict a method for proteomic analysis by "one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis" (1D SDS-PAGE) and a sample preparation procedure for "liquid chromatography in tandem with mass spectrometry" (LC-MS/MS), and we present some generic points to consider during LC-MS/MS.Entities:
Keywords: 1D SDS-PAGE; LC-MS/MS; RNA-seq; Real-time qPCR; Toxicogenomics
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Year: 2021 PMID： 33423233 DOI： 10.1007/978-1-0716-1091-6_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745