Literature DB >> 33421012

Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue.

Omid Azimzadeh1, Michael J Atkinson2,3, Soile Tapio2.   

Abstract

Clinical tissue archives represent an invaluable source of biological information. Formalin-fixed, paraffin-embedded (FFPE) tissue can be used for retrospective investigation of biomarkers of diseases and prognosis.Recently, the number of studies using proteome profiling of samples from clinical archives has markedly increased. However, the application of conventional quantitative proteomics technologies remains a challenge mainly due to the harsh fixation process resulting in protein cross-linking and protein degradation. In the present chapter, we demonstrate a protocol for label-free proteomic analysis of FFPE tissue prepared from human cardiac autopsies. The data presented here highlight the applicability and suitability of FFPE heart tissue for understanding the molecular mechanism of cardiac injury using a proteomics approach.

Entities:  

Keywords:  Cross-linking; Formalin-fixed, paraffin-embedded (FFPE); Heart; Label-free; Protein extraction; Proteomics

Mesh：

Substances：

Year:  2021        PMID： 33421012     DOI： 10.1007/978-1-0716-1186-9_33

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Formalin-fixed paraffin-embedded (FFPE) proteome analysis using gel-free and gel-based proteomics.

Authors:  Omid Azimzadeh; Zarko Barjaktarovic; Michaela Aubele; Julia Calzada-Wack; Hakan Sarioglu; Michael J Atkinson; Soile Tapio
Journal:  J Proteome Res       Date:  2010-09-03       Impact factor: 4.466

2.  Universal sample preparation method for proteome analysis.

Authors:  Jacek R Wiśniewski; Alexandre Zougman; Nagarjuna Nagaraj; Matthias Mann
Journal:  Nat Methods       Date:  2009-04-19       Impact factor: 28.547

3.  Progress in updating the European Radiobiology Archives.

Authors:  S Tapio; P N Schofield; C Adelmann; M J Atkinson; J L B Bard; H Bijwaard; M Birschwilks; P Dubus; L Fiette; G Gerber; M Gruenberger; L Quintanilla-Martinez; B Rozell; S Saigusa; M Warren; C R Watson; B Grosche
Journal:  Int J Radiat Biol       Date:  2008-11       Impact factor: 2.694

Review 4.  Use of proteomics in radiobiological research: current state of the art.

Authors:  Soile Tapio; Sabine Hornhardt; Maria Gomolka; Dariusz Leszczynski; Anton Posch; Stefan Thalhammer; Michael J Atkinson
Journal:  Radiat Environ Biophys       Date:  2010-01-05       Impact factor: 1.925

Review 5.  Update on proteomic studies of formalin-fixed paraffin-embedded tissues.

Authors:  Laura Giusti; Cristina Angeloni; Antonio Lucacchini
Journal:  Expert Rev Proteomics       Date:  2019-05-16       Impact factor: 3.940

Review 6.  Proteomic developments in the analysis of formalin-fixed tissue.

Authors:  Ove J R Gustafsson; Georgia Arentz; Peter Hoffmann
Journal:  Biochim Biophys Acta       Date:  2014-10-12

7.  Proteomic workflow for analysis of archival formalin-fixed and paraffin-embedded clinical samples to a depth of 10 000 proteins.

Authors:  Jacek R Wiśniewski; Kamila Duś; Matthias Mann
Journal:  Proteomics Clin Appl       Date:  2013-03-06       Impact factor: 3.494

Review 8.  Proteomic studies of formalin-fixed paraffin-embedded tissues.

Authors:  Laura Giusti; Antonio Lucacchini
Journal:  Expert Rev Proteomics       Date:  2013-04       Impact factor: 3.940

Review 9.  Applications of mass spectrometry for quantitative protein analysis in formalin-fixed paraffin-embedded tissues.

Authors:  Carine Steiner; Axel Ducret; Jean-Christophe Tille; Marlene Thomas; Thomas A McKee; Laura Rubbia-Brandt; Alexander Scherl; Pierre Lescuyer; Paul Cutler
Journal:  Proteomics       Date:  2014-03       Impact factor: 3.984

10.  High-throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification.

Authors:  Yi Zhu; Tobias Weiss; Qiushi Zhang; Rui Sun; Bo Wang; Xiao Yi; Zhicheng Wu; Huanhuan Gao; Xue Cai; Guan Ruan; Tiansheng Zhu; Chao Xu; Sai Lou; Xiaoyan Yu; Ludovic Gillet; Peter Blattmann; Karim Saba; Christian D Fankhauser; Michael B Schmid; Dorothea Rutishauser; Jelena Ljubicic; Ailsa Christiansen; Christine Fritz; Niels J Rupp; Cedric Poyet; Elisabeth Rushing; Michael Weller; Patrick Roth; Eugenia Haralambieva; Silvia Hofer; Chen Chen; Wolfram Jochum; Xiaofei Gao; Xiaodong Teng; Lirong Chen; Qing Zhong; Peter J Wild; Ruedi Aebersold; Tiannan Guo
Journal:  Mol Oncol       Date:  2019-09-18       Impact factor: 6.603
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