| Literature DB >> 33420994 |
Jonathan M Preuss1, Ute Burret2, Sabine Vettorazzi3.
Abstract
The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA). Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes. Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format. This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition. Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.Entities:
Keywords: Bead-based; Bioplex; Chemokine; Cytokine; Endotoxemia; Glucocorticoid receptor; Inflammation; Intensive care; Multiplex; Serum
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Year: 2021 PMID： 33420994 DOI： 10.1007/978-1-0716-1186-9_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745