| Literature DB >> 33420022 |
Hin Chu1,2, Bingjie Hu2, Xiner Huang2, Yue Chai2, Dongyan Zhou2, Yixin Wang2, Huiping Shuai2, Dong Yang2, Yuxin Hou2, Xi Zhang2, Terrence Tsz-Tai Yuen2, Jian-Piao Cai2, Anna Jinxia Zhang1,2, Jie Zhou1,2, Shuofeng Yuan1,2, Kelvin Kai-Wang To1,2,3,4, Ivy Hau-Yee Chan5, Ko-Yung Sit5, Dominic Chi-Chung Foo5, Ian Yu-Hong Wong5, Ada Tsui-Lin Ng5, Tan To Cheung5, Simon Ying-Kit Law5, Wing-Kuk Au5, Melinda A Brindley6, Zhiwei Chen1,2,3, Kin-Hang Kok1,2,3, Jasper Fuk-Woo Chan7,8,9,10,11, Kwok-Yung Yuen12,13,14,15,16.
Abstract
Understanding the factors that contribute to efficient SARS-CoV-2 infection of human cells may provide insights on SARS-CoV-2 transmissibility and pathogenesis, and reveal targets of intervention. Here, we analyze host and viral determinants essential for efficient SARS-CoV-2 infection in both human lung epithelial cells and ex vivo human lung tissues. We identify heparan sulfate as an important attachment factor for SARS-CoV-2 infection. Next, we show that sialic acids present on ACE2 prevent efficient spike/ACE2-interaction. While SARS-CoV infection is substantially limited by the sialic acid-mediated restriction in both human lung epithelial cells and ex vivo human lung tissues, infection by SARS-CoV-2 is limited to a lesser extent. We further demonstrate that the furin-like cleavage site in SARS-CoV-2 spike is required for efficient virus replication in human lung but not intestinal tissues. These findings provide insights on the efficient SARS-CoV-2 infection of human lungs.Entities:
Year: 2021 PMID： 33420022 DOI： 10.1038/s41467-020-20457-w
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919