Manuel Toledano-Osorio1, Francisco Javier Manzano-Moreno2, Concepción Ruiz3, Manuel Toledano4, Raquel Osorio5. 1. Biomaterials in Dentistry Research Group, Department of Stomatology, School of Dentistry, University of Granada, Spain; Medicina Clínica y Salud Pública PhD Programme, Spain. 2. Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs. Granada, Granada, Spain. 3. Instituto Investigación Biosanitaria, ibs. Granada, Granada, Spain; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences. University of Granada, Spain; Institute of Neuroscience, University of Granada, Centro de Investigación Biomédica (CIBM), Parque Tecnológico de la Salud (PTS), Granada, Spain. 4. Biomaterials in Dentistry Research Group, Department of Stomatology, School of Dentistry, University of Granada, Spain. Electronic address: toledano@ugr.es. 5. Biomaterials in Dentistry Research Group, Department of Stomatology, School of Dentistry, University of Granada, Spain.
Abstract
OBJECTIVES: Maxillofacial bone defects are the main hindering conditions for traditional dental implant strategies. Guided Bone Regeneration (GBR) is used to handle this situation. The principle of GBR is to use a membrane to prevent the colonization of soft tissue cells of the bone defect and favors the migration of osteogenic linages. Current membranes do not completely fulfill the requirements that an optimal membrane should have, sometimes resulting in non-predictable results. Thus, the need to develop an ideal membrane to perform this duty is clear. Recent developments in bio-manufacturing are driving innovations in membranes technology permitting the active participation of the membrane in the healing and regenerative process trough native tissue mimicking, drug-delivery and cells interaction, away from being a passive barrier. New membranes features need specific evaluation techniques, beyond the International Standard for membrane materials (last reviewed in 2004), being this the rationale for the present review. Nanotechnology application has completely shifted the way of analyzing structural characterization. New progresses on osteoimmmunomodulation have also switched the understanding of cells-membranes interaction. DATA AND SOURCES: To propose an updated protocol for GBR membranes evaluation, critical reading of the relevant published literature was carried out after a MEDLINE/PubMed database search. CONCLUSIONS: The main findings are that a potential active membrane should be assessed in its nanostructure, physicochemical and nanomechanical properties, bioactivity and antibacterial, osteoblasts proliferation, differentiation and mineralization. Immunomodulation testing for macrophages recruitment and M2 phenotype promotion in osteoblasts co-culture has to be achieved to completely analyze membranes/tissue interactions.
OBJECTIVES: Maxillofacial bone defects are the main hindering conditions for traditional dental implant strategies. Guided Bone Regeneration (GBR) is used to handle this situation. The principle of GBR is to use a membrane to prevent the colonization of soft tissue cells of the bone defect and favors the migration of osteogenic linages. Current membranes do not completely fulfill the requirements that an optimal membrane should have, sometimes resulting in non-predictable results. Thus, the need to develop an ideal membrane to perform this duty is clear. Recent developments in bio-manufacturing are driving innovations in membranes technology permitting the active participation of the membrane in the healing and regenerative process trough native tissue mimicking, drug-delivery and cells interaction, away from being a passive barrier. New membranes features need specific evaluation techniques, beyond the International Standard for membrane materials (last reviewed in 2004), being this the rationale for the present review. Nanotechnology application has completely shifted the way of analyzing structural characterization. New progresses on osteoimmmunomodulation have also switched the understanding of cells-membranes interaction. DATA AND SOURCES: To propose an updated protocol for GBR membranes evaluation, critical reading of the relevant published literature was carried out after a MEDLINE/PubMed database search. CONCLUSIONS: The main findings are that a potential active membrane should be assessed in its nanostructure, physicochemical and nanomechanical properties, bioactivity and antibacterial, osteoblasts proliferation, differentiation and mineralization. Immunomodulation testing for macrophages recruitment and M2 phenotype promotion in osteoblasts co-culture has to be achieved to completely analyze membranes/tissue interactions.
Authors: Yilin Shi; Jin Liu; Mi Du; Shengben Zhang; Yue Liu; Hu Yang; Ruiwen Shi; Yuanyuan Guo; Feng Song; Yajun Zhao; Jing Lan Journal: Front Bioeng Biotechnol Date: 2022-06-28
Authors: Manuel Toledano; Marta Vallecillo-Rivas; María T Osorio; Esther Muñoz-Soto; Manuel Toledano-Osorio; Cristina Vallecillo; Raquel Toledano; Christopher D Lynch; María-Angeles Serrera-Figallo; Raquel Osorio Journal: Polymers (Basel) Date: 2021-05-29 Impact factor: 4.329
Authors: Manuel Toledano-Osorio; Francisco J Manzano-Moreno; Manuel Toledano; Antonio L Medina-Castillo; Victor J Costela-Ruiz; Concepción Ruiz; Raquel Osorio Journal: Polymers (Basel) Date: 2021-03-28 Impact factor: 4.329