Ourania I Koukou1, Lampros D Pappas2, Pelagia Chloropoulou3, Maria A Kouroupi4, Konstantinos I Koukos5, Georgia Karpathiou6, Antonios A Galanos7, Georgios I Drosos8, Evaggelos Magnisalis9, Alexandra N Giatromanolaki4, Dionysios Alexandros Verettas10. 1. Private Practice, Komotini 69132, Greece. 2. Department of Orthopaedics, General Hospital of Didymoteicho, Didymoteicho 68300, Greece. 3. Department of Anesthesiology, School of Medicine, Democritus University of Thrace, Alexandroupolis 68100, Greece. 4. Department of Pathology, School of Medicine, Democritus University of Thrace, Alexandroupolis 68100, Greece. 5. Department of Orthopaedics, General Hospital of Preveza, 48100, Greece. 6. Department of Pathology, University Hospital of Saint-Etienne, 42055 CEDEX2 Saint-Etienne, France. 7. Laboratory of Research of the Musculoskeletal System, School of Medicine University of Athens, 14561, Greece. 8. Department of Orthopaedics, School of Medicine, Democritus University of Thrace, Alexandroupolis 68100, Greece. 9. Laboratory of Bioengineering, BioHexagon Ltd, Athens 17124, Greece. 10. Democritus University of Thrace, Alexandroupolis 68100, Greece.
Abstract
BACKGROUND: Strontium ranelate (StR) is an antiosteoporotic agent previously utilized for the enhancement of fracture union. We investigated the effects of StR on fracture healing using a rabbit model. METHODS: Forty adult female rabbits were included in the study and were divided in 2 equal groups, according to StR treatment or untreated controls. All animals were subjected to osteotomy of the ulna, while the contralateral ulna remained intact and served as a control for the biomechanical assessment of fracture healing. Animals in the study group received 600 mg/kg/day of StR orally. All animals received ordinary food. At 2 and 4 weeks, all animals were euthanatized and the osteotomy sites were evaluated for healing through radiological, biomechanical, and histopathological studies. RESULTS: The treatment group presented statistically significant higher callus diameter, total callus area, percentage of fibrous tissue (p < 0.001), vessels/mm2, number of total vessels, and lower osteoclast number/mm2 (p < 0.05) than the control group at 2 weeks. Additionally, the treatment group presented significantly higher percentages of new trabecular bone, vessels/mm2, osteoclast number/mm2, and lower values for callus diameter, as well as total callus area (p < 0.05), than the control group at 4 weeks. At 4 weeks, in the treatment group, force applied (p = 0.003), energy at failure (p = 0.004), and load at failure (p = 0.003) were all significantly higher in the forearm specimens with the osteotomized ulnae compared to those without. Radiological bone union was demonstrated for animals receiving StR at 4 weeks compared with controls (p = 0.045). CONCLUSION: StR appears to enhance fracture healing but further studies are warranted in order to better elucidate the mechanisms and benefits of StR treatment.
BACKGROUND: Strontium ranelate (StR) is an antiosteoporotic agent previously utilized for the enhancement of fracture union. We investigated the effects of StR on fracture healing using a rabbit model. METHODS: Forty adult female rabbits were included in the study and were divided in 2 equal groups, according to StR treatment or untreated controls. All animals were subjected to osteotomy of the ulna, while the contralateral ulna remained intact and served as a control for the biomechanical assessment of fracture healing. Animals in the study group received 600 mg/kg/day of StR orally. All animals received ordinary food. At 2 and 4 weeks, all animals were euthanatized and the osteotomy sites were evaluated for healing through radiological, biomechanical, and histopathological studies. RESULTS: The treatment group presented statistically significant higher callus diameter, total callus area, percentage of fibrous tissue (p < 0.001), vessels/mm2, number of total vessels, and lower osteoclast number/mm2 (p < 0.05) than the control group at 2 weeks. Additionally, the treatment group presented significantly higher percentages of new trabecular bone, vessels/mm2, osteoclast number/mm2, and lower values for callus diameter, as well as total callus area (p < 0.05), than the control group at 4 weeks. At 4 weeks, in the treatment group, force applied (p = 0.003), energy at failure (p = 0.004), and load at failure (p = 0.003) were all significantly higher in the forearm specimens with the osteotomized ulnae compared to those without. Radiological bone union was demonstrated for animals receiving StR at 4 weeks compared with controls (p = 0.045). CONCLUSION: StR appears to enhance fracture healing but further studies are warranted in order to better elucidate the mechanisms and benefits of StR treatment.
Authors: G Boivin; P Deloffre; B Perrat; G Panczer; M Boudeulle; Y Mauras; P Allain; Y Tsouderos; P J Meunier Journal: J Bone Miner Res Date: 1996-09 Impact factor: 6.741
Authors: R K Fuchs; M R Allen; K W Condon; S Reinwald; L M Miller; D McClenathan; B Keck; R J Phipps; D B Burr Journal: Osteoporos Int Date: 2008-04-03 Impact factor: 5.071