Yunying Zhou1,2,3, Jason J Chen4,5. 1. Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. 2. Medical Research & Laboratory Diagnostic Center, Central Hospital Affiliated To Shandong First Medical University, Jinan, China. 3. The Cancer Research Center, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. 4. Department of Microbiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. jxchen@sdu.edu.cn. 5. The Cancer Research Center, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. jxchen@sdu.edu.cn.
Abstract
BACKGROUND: Signal transducers and activators of transcription 3 (STAT3) is a transcription factor that plays a key role in many cellular processes such as cell growth and cancer. However, the functions and mechanisms by which STAT3 regulates cellular processes are not fully understood. RESULTS: Here we describe a novel function of STAT3. We demonstrated that STAT3 plays an important role in DNA replication. Specifically, knockdown of STAT3 reduced DNA replication while activation and ectopic expression of STAT3 promoted DNA replication. We further identified the WD repeat and HMG-box DNA-binding protein 1 (WDHD1), which plays an important role in DNA replication initiation, as a novel STAT3 target gene that mediated the DNA replication function of STAT3. We showed that STAT3 bind the promoter/up regulatory region of WDHD1 gene. CONCLUSIONS: These studies identified a novel function of STAT3 that is mediated by its newly identified target gene WDHD1 and have important implications.
BACKGROUND:Signal transducers and activators of transcription 3 (STAT3) is a transcription factor that plays a key role in many cellular processes such as cell growth and cancer. However, the functions and mechanisms by which STAT3 regulates cellular processes are not fully understood. RESULTS: Here we describe a novel function of STAT3. We demonstrated that STAT3 plays an important role in DNA replication. Specifically, knockdown of STAT3 reduced DNA replication while activation and ectopic expression of STAT3 promoted DNA replication. We further identified the WD repeat and HMG-box DNA-binding protein 1 (WDHD1), which plays an important role in DNA replication initiation, as a novel STAT3 target gene that mediated the DNA replication function of STAT3. We showed that STAT3 bind the promoter/up regulatory region of WDHD1 gene. CONCLUSIONS: These studies identified a novel function of STAT3 that is mediated by its newly identified target gene WDHD1 and have important implications.
Entities:
Keywords:
DNA replication; STAT3; Transcription; WDHD1
Authors: K Takeda; K Noguchi; W Shi; T Tanaka; M Matsumoto; N Yoshida; T Kishimoto; S Akira Journal: Proc Natl Acad Sci U S A Date: 1997-04-15 Impact factor: 11.205
Authors: Daniel J Gough; Alicia Corlett; Karni Schlessinger; Joanna Wegrzyn; Andrew C Larner; David E Levy Journal: Science Date: 2009-06-26 Impact factor: 47.728