Joseneide Viana de Almeida1, Cristian Ferreira de Souza2, Andressa Alencastre Fuzari2, Christie A Joya3, Hugo O Valdivia3, Daniella Castanheira Bartholomeu4, Reginaldo Peçanha Brazil5. 1. Programa de Pós-Graduação em Biologia Parasitária, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. 2. Laboratório de Doenças Parasitárias, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. 3. Department of Parasitology, US Naval Medical Research Unit 6, Lima, Peru. 4. Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. 5. Laboratório de Doenças Parasitárias, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. brazil.reginaldo@gmail.com.
Abstract
BACKGROUND: Cutaneous leishmaniasis (CL) is an endemic disease in Brazil that is highly prevalent in the northern region of the country. Although there is a continuous and growing number of cases registered in the state of Roraima, there is limited information regarding the species of Leishmania that affect the human population. In this study, we aimed to characterize which Leishmania species cause human disease in those presenting with cutaneous leishmaniasis in endemic areas of the State of Roraima. METHODS: We conducted a prospective surveillance study between 2016 to 2018 in health centers located in the State of Roraima, Brazil. Participants with clinical suspicion of CL were enrolled and provided lesion samples for parasitological detection by microscopy. A subset of the samples was tested by polymerase chain reaction and sequencing of the internal transcribed spacer 1 (ITS-1 PCR) for molecular species identification. RESULTS: A total of 262 participants were enrolled in this study. Of those, 129 (49.27%) were positive by parasitological examination. Most positive subjects (81.58%) were male, and most cases presented a single lesion (80.26%). ITS-1 PCR and sequencing on a subset of 76 samples allowed us to detect nine different species of Leishmania: L. (V.) braziliensis, L (V.) panamensis, L. (V.) guyanensis, L. (V.) naiffi, L. (V.) shawi, L.(V.) utingensis, L. (V.) lindenbergi, L. (L.) amazonensis and L. (L.) mexicana. CONCLUSIONS: Our study provides the first assessment of circulating species of Leishmania in the State of Roraima, Brazil, and shows the high diversity in this region. This study opens the path for further research on the transmission of leishmaniasis in the northernmost Brazilian State including vector and reservoir surveillance as well as for intensification of investigation and control activities against CL in the region.
BACKGROUND:Cutaneous leishmaniasis (CL) is an endemic disease in Brazil that is highly prevalent in the northern region of the country. Although there is a continuous and growing number of cases registered in the state of Roraima, there is limited information regarding the species of Leishmania that affect the human population. In this study, we aimed to characterize which Leishmania species cause human disease in those presenting with cutaneous leishmaniasis in endemic areas of the State of Roraima. METHODS: We conducted a prospective surveillance study between 2016 to 2018 in health centers located in the State of Roraima, Brazil. Participants with clinical suspicion of CL were enrolled and provided lesion samples for parasitological detection by microscopy. A subset of the samples was tested by polymerase chain reaction and sequencing of the internal transcribed spacer 1 (ITS-1 PCR) for molecular species identification. RESULTS: A total of 262 participants were enrolled in this study. Of those, 129 (49.27%) were positive by parasitological examination. Most positive subjects (81.58%) were male, and most cases presented a single lesion (80.26%). ITS-1 PCR and sequencing on a subset of 76 samples allowed us to detect nine different species of Leishmania: L. (V.) braziliensis, L (V.) panamensis, L. (V.) guyanensis, L. (V.) naiffi, L. (V.) shawi, L.(V.) utingensis, L. (V.) lindenbergi, L. (L.) amazonensis and L. (L.) mexicana. CONCLUSIONS: Our study provides the first assessment of circulating species of Leishmania in the State of Roraima, Brazil, and shows the high diversity in this region. This study opens the path for further research on the transmission of leishmaniasis in the northernmost Brazilian State including vector and reservoir surveillance as well as for intensification of investigation and control activities against CL in the region.
Authors: Luciana P Oliveira; Luciana C S Nascimento; Fabiola S Santos; Jaqueline L C Takamatsu; Luiz R P Sanchez; Walter S Santos; Lourdes M Garcez Journal: Int J Environ Res Public Health Date: 2022-05-23 Impact factor: 4.614
Authors: Emma Taylor; Elsa Gladys Aguilar-Ancori; Ashley C Banyard; Isis Abel; Clara Mantini-Briggs; Charles L Briggs; Carolina Carrillo; Cesar M Gavidia; Ricardo Castillo-Neyra; Alejandro D Parola; Fredy E Villena; Joaquin M Prada; Brett W Petersen; Nestor Falcon Perez; Cesar Cabezas Sanchez; Moises Sihuincha; Daniel G Streicker; Ciro Maguina Vargas; Ana Maria Navarro Vela; Marco A N Vigilato; Hui Wen Fan; Rodney Willoughby; Daniel L Horton; Sergio E Recuenco Journal: Int Health Date: 2022-07-27 Impact factor: 3.131