BACKGROUND: Psychrotrophic lactic acid bacteria (LAB) species are the dominant species in the microbiota of cold-stored modified-atmosphere-packaged food products and are the main cause of food spoilage. Despite the importance of psychrotrophic LAB, their response to cold or heat has not been studied. Here, we studied the transcriptome-level cold- and heat-shock response of spoilage lactic acid bacteria with time-series RNA-seq for Le. gelidum, Lc. piscium, and P. oligofermentans at 0 °C, 4 °C, 14 °C, 25 °C, and 28 °C. RESULTS: We observed that the cold-shock protein A (cspA) gene was the main cold-shock protein gene in all three species. Our results indicated that DEAD-box RNA helicase genes (cshA, cshB) also play a critical role in cold-shock response in psychrotrophic LAB. In addition, several RNase genes were involved in cold-shock response in Lc. piscium and P. oligofermentans. Moreover, gene network inference analysis provided candidate genes involved in cold-shock response. Ribosomal proteins, tRNA modification, rRNA modification, and ABC and efflux MFS transporter genes clustered with cold-shock response genes in all three species, indicating that these genes could be part of the cold-shock response machinery. Heat-shock treatment caused upregulation of Clp protease and chaperone genes in all three species. We identified transcription binding site motifs for heat-shock response genes in Le. gelidum and Lc. piscium. Finally, we showed that food spoilage-related genes were upregulated at cold temperatures. CONCLUSIONS: The results of this study provide new insights on the cold- and heat-shock response of psychrotrophic LAB. In addition, candidate genes involved in cold- and heat-shock response predicted using gene network inference analysis could be used as targets for future studies.
BACKGROUND: Psychrotrophic lactic acid bacteria (LAB) species are the dominant species in the microbiota of cold-stored modified-atmosphere-packaged food products and are the main cause of food spoilage. Despite the importance of psychrotrophic LAB, their response to cold or heat has not been studied. Here, we studied the transcriptome-level cold- and heat-shock response of spoilage lactic acid bacteria with time-series RNA-seq for Le. gelidum, Lc. piscium, and P. oligofermentans at 0 °C, 4 °C, 14 °C, 25 °C, and 28 °C. RESULTS: We observed that the cold-shock protein A (cspA) gene was the main cold-shock protein gene in all three species. Our results indicated that DEAD-box RNA helicase genes (cshA, cshB) also play a critical role in cold-shock response in psychrotrophic LAB. In addition, several RNase genes were involved in cold-shock response in Lc. piscium and P. oligofermentans. Moreover, gene network inference analysis provided candidate genes involved in cold-shock response. Ribosomal proteins, tRNA modification, rRNA modification, and ABC and efflux MFS transporter genes clustered with cold-shock response genes in all three species, indicating that these genes could be part of the cold-shock response machinery. Heat-shock treatment caused upregulation of Clp protease and chaperone genes in all three species. We identified transcription binding site motifs for heat-shock response genes in Le. gelidum and Lc. piscium. Finally, we showed that food spoilage-related genes were upregulated at cold temperatures. CONCLUSIONS: The results of this study provide new insights on the cold- and heat-shock response of psychrotrophic LAB. In addition, candidate genes involved in cold- and heat-shock response predicted using gene network inference analysis could be used as targets for future studies.
Authors: Riitta Rahkila; Timo Nieminen; Per Johansson; Elina Säde; Johanna Björkroth Journal: Int J Food Microbiol Date: 2012-03-06 Impact factor: 5.277
Authors: T T Nieminen; K Koskinen; P Laine; J Hultman; E Säde; L Paulin; A Paloranta; P Johansson; J Björkroth; P Auvinen Journal: Int J Food Microbiol Date: 2012-04-29 Impact factor: 5.277
Authors: K J Björkroth; R Geisen; U Schillinger; N Weiss; P De Vos; W H Holzapfel; H J Korkeala; P Vandamme Journal: Appl Environ Microbiol Date: 2000-09 Impact factor: 4.792
Authors: Elina Jääskeläinen; Per Johansson; Olli Kostiainen; Timo Nieminen; Georg Schmidt; Panu Somervuo; Marzia Mohsina; Paula Vanninen; Petri Auvinen; Johanna Björkroth Journal: Appl Environ Microbiol Date: 2012-11-30 Impact factor: 4.792
Authors: Javier Feito; Diogo Contente; Manuel Ponce-Alonso; Lara Díaz-Formoso; Carlos Araújo; Nuria Peña; Juan Borrero; Beatriz Gómez-Sala; Rosa Del Campo; Estefanía Muñoz-Atienza; Pablo E Hernández; Luis M Cintas Journal: Microorganisms Date: 2022-02-28