| Literature DB >> 3341112 |
J Donson1, H V Gunn, C J Woolston, M S Pinner, M I Boulton, P M Mullineaux, J W Davies.
Abstract
A monomeric clone of double-stranded DNA synthesized in vitro DNA of the geminivirus Digitaria streak (DSV) was subcloned as a tandem dimeric unit into a binary vector of Agrobacterium tumefaciens, creating a plasmid pDS2. Inoculation of digitaria sanguinalis with A. tumefaciens carrying pDS2 resulted in viral infection. The symptoms, virus particles, and DNA forms obtained were indistinguishable from those of a natural DSV infection of D. sanguinalis. Inoculations have also induced infections in Zea mays and Avena sativa. The sequence of the Agrobacterium-mediated infectious clone of DSV has been determined.Entities:
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Year: 1988 PMID: 3341112 DOI: 10.1016/0042-6822(88)90416-3
Source DB: PubMed Journal: Virology ISSN: 0042-6822 Impact factor: 3.616