Stephan Payr1,2, Elizabeth Rosado-Balmayor3,4, Thomas Tiefenboeck5, Tim Schuseil3, Marina Unger3, Claudine Seeliger3, Martijn van Griensven3,6. 1. Department of Experimental Trauma Surgery, Klinikum Rechts der Isar, Technical University Munich, Munich, Germany. stephan.payr@meduniwien.ac.at. 2. Department of Orthopedics and Trauma Surgery, Division of Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. stephan.payr@meduniwien.ac.at. 3. Department of Experimental Trauma Surgery, Klinikum Rechts der Isar, Technical University Munich, Munich, Germany. 4. Department IBE, MERLN Institute, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, The Netherlands. 5. Department of Orthopedics and Trauma Surgery, Division of Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. 6. Department cBITE, MERLN Institute, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, The Netherlands.
Abstract
BACKGROUND: The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. METHODS: Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. RESULTS: Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). CONCLUSION: Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. TRIAL REGISTRATION: 5217/11 on the 22nd of Dec. 2011.
BACKGROUND: The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. METHODS: Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. RESULTS:Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). CONCLUSION: Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. TRIAL REGISTRATION: 5217/11 on the 22nd of Dec. 2011.
Authors: Jane P Dillon; Victoria J Waring-Green; Adam M Taylor; Peter J M Wilson; Mark Birch; Alison Gartland; James A Gallagher Journal: Methods Mol Biol Date: 2012
Authors: Christoph Pautke; Matthias Schieker; Thomas Tischer; Andreas Kolk; Peter Neth; Wolf Mutschler; Stefan Milz Journal: Anticancer Res Date: 2004 Nov-Dec Impact factor: 2.480
Authors: Guiti Z Eghbali-Fatourechi; Ulrike I L Mödder; Natthinee Charatcharoenwitthaya; Arunik Sanyal; Anita H Undale; Jackie A Clowes; James E Tarara; Sundeep Khosla Journal: Bone Date: 2007-01-04 Impact factor: 4.398
Authors: Louis C Gerstenfeld; Dennis M Cullinane; George L Barnes; Dana T Graves; Thomas A Einhorn Journal: J Cell Biochem Date: 2003-04-01 Impact factor: 4.429
Authors: Robert J Pignolo; Robin K Suda; Emily A McMillan; Johnny Shen; Seoung-Hoon Lee; Yongwon Choi; Alexander C Wright; F Brad Johnson Journal: Aging Cell Date: 2007-11-20 Impact factor: 9.304
Authors: Shelly Arora; Paul R Cooper; Jithendra T Ratnayake; Lara T Friedlander; Shakila B Rizwan; Benedict Seo; Haizal M Hussaini Journal: Int Endod J Date: 2022-02-04 Impact factor: 5.165