Huixin Hu1,2,3, Jingjing Jing1,2,3, Xiaodong Lu1,2,3, Yuan Yuan4,5,6, Chengzhong Xing7,8,9. 1. Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University, 155# North Nanjing Street, Heping District, Shenyang City, Liaoning Province, 110001, China. 2. Liaoning Provincial Education Department, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Shenyang, 110001, China. 3. Key Laboratory of Gastrointestinal Cancer Etiology and Screening, Shenyang, 110001, Liaoning Province, China. 4. Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University, 155# North Nanjing Street, Heping District, Shenyang City, Liaoning Province, 110001, China. yuanyuan@cmu.edu.cn. 5. Liaoning Provincial Education Department, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Shenyang, 110001, China. yuanyuan@cmu.edu.cn. 6. Key Laboratory of Gastrointestinal Cancer Etiology and Screening, Shenyang, 110001, Liaoning Province, China. yuanyuan@cmu.edu.cn. 7. Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University, 155# North Nanjing Street, Heping District, Shenyang City, Liaoning Province, 110001, China. xcz1966@126.com. 8. Liaoning Provincial Education Department, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Shenyang, 110001, China. xcz1966@126.com. 9. Key Laboratory of Gastrointestinal Cancer Etiology and Screening, Shenyang, 110001, Liaoning Province, China. xcz1966@126.com.
Abstract
BACKGROUND: XPF (xeroderma pigmentosum complementation group F) is a key factor contributing to DNA damage excision of nucleotide excision repair pathway. The relationship between XPF expression and the risk and prognosis of colorectal cancer (CRC) is unclear. METHODS: In this experiment, a total of 824 cases of colorectal tissue were collected. XPF protein expression was detected by immunohistochemical staining. We conducted a Mann-Whitney U test in order to explore the differential expression of XPF between CRC and non-cancer controls, and the correlation between XPF expression and CRC clinicopathological parameters. Univariate and multivariate Cox regression analyses were conducted to investigate the relationship between XPF expression and CRC prognosis. The Java based software GSEA as well as STRING, David, GO, KEGG were used to explore the function and regulation network of XPF. RESULTS: The results demonstrated that the XPF expression in CRC was significantly up-regulated compared with non-tumor controls (P < 0.001) and adenoma tissue (P < 0.001). XPF protein was increased in the dynamic sequence of anal diseases to adenoma tissue to CRC. Expression of XPF was related to tumor location (P = 0.005) and tumor growth pattern (P = 0.009). The results of prognosis analysis suggested that in patients with stage T1-T2, XPF low expression may be significantly associated with better overall survival (HR = 7.978, 95% CI 1.208-52.673, P = 0.031). XPF and its interacting genes played a vital role in different processes of nucleotide excision repair pathway. XPF expression was related with Ubiquitin like protein specific protease activity. CONCLUSIONS: XPF might be a promising biomarker for CRC risk, and also showed potential as a prognostic predictor in CRC patients.
BACKGROUND:XPF (xeroderma pigmentosum complementation group F) is a key factor contributing to DNA damage excision of nucleotide excision repair pathway. The relationship between XPF expression and the risk and prognosis of colorectal cancer (CRC) is unclear. METHODS: In this experiment, a total of 824 cases of colorectal tissue were collected. XPF protein expression was detected by immunohistochemical staining. We conducted a Mann-Whitney U test in order to explore the differential expression of XPF between CRC and non-cancer controls, and the correlation between XPF expression and CRC clinicopathological parameters. Univariate and multivariate Cox regression analyses were conducted to investigate the relationship between XPF expression and CRC prognosis. The Java based software GSEA as well as STRING, David, GO, KEGG were used to explore the function and regulation network of XPF. RESULTS: The results demonstrated that the XPF expression in CRC was significantly up-regulated compared with non-tumor controls (P < 0.001) and adenoma tissue (P < 0.001). XPF protein was increased in the dynamic sequence of anal diseases to adenoma tissue to CRC. Expression of XPF was related to tumor location (P = 0.005) and tumor growth pattern (P = 0.009). The results of prognosis analysis suggested that in patients with stage T1-T2, XPF low expression may be significantly associated with better overall survival (HR = 7.978, 95% CI 1.208-52.673, P = 0.031). XPF and its interacting genes played a vital role in different processes of nucleotide excision repair pathway. XPF expression was related with Ubiquitin like protein specific protease activity. CONCLUSIONS:XPF might be a promising biomarker for CRC risk, and also showed potential as a prognostic predictor in CRC patients.
Authors: Alec Vaezi; Xiaozhe Wang; Shama Buch; William Gooding; Lin Wang; Raja R Seethala; David T Weaver; Alan D D'Andrea; Athanassios Argiris; Marjorie Romkes; Laura J Niedernhofer; Jennifer R Grandis Journal: Clin Cancer Res Date: 2011-07-07 Impact factor: 12.531
Authors: Jana Slyskova; Vlasta Korenkova; Andrew R Collins; Pavel Prochazka; Ludmila Vodickova; Jiri Svec; Ludmila Lipska; Miroslav Levy; Michaela Schneiderova; Vaclav Liska; Lubos Holubec; Rajiv Kumar; Pavel Soucek; Alessio Naccarati; Pavel Vodicka Journal: Clin Cancer Res Date: 2012-09-10 Impact factor: 12.531