Amir Sohrabi1, Fatemeh Bassam-Tolami2, Mohsen Imani3. 1. Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Nobels väg 12A, Solna Campus, PO Box 171 77, Stockholm, Sweden. 2. Department of Biology, Tehran Shargh Branch, Payame Noor University, Tehran, Iran. 3. Department of Molecular Biology, Research Center of Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Abstract
BACKGROUND: HPV genotypes are the most common etiological factor for genital neoplasia. It would appear that sexually transmitted infections accompanied with HPV genotypes might have synergistic interactions in cancer progression. The genetic polymorphisms are involved in metabolizing carcinogens which may contribute to the susceptibility of developing genital cancers by less efficient or overly down metabolic pathways and cell signaling. MTHFR polymorphisms are related to several metabolic disorders and human cancers. We investigated the contribution of MTHFR 1298 and MTHFR 677 polymorphisms as potential risk factors for outcomes with HPV genotypes and STIs in Iranian population. MATERIALS AND METHODS: As a case-control study, MTHFR A1298C and C677T were assessed for SNPs analysis using a PCR-RFLP assay in 50 cervical intraepithelial neoplasia (CIN) cases, 98 HPV-positive subjects and 47 non-cancerous/non-HPV patients as healthy controls. RESULTS: Finding suggested a significant association between the MTHFR 1298 CC polymorphisms (OR = 3.5, 95% CI = 1.13-10.82, P ≤ 0.05) in women with CIN as compared to non-cancerous/non-HPV subjects. There was not a significant difference of MTHFR 677 between outcomes. DISCUSSION: It would seem MTHFR 1298 CC is more likely to be a potential risk factor for HPV-cervical cancer progression. Consequences support further attempts to understand the clinical manifestations of neoplasia related to genital infections and gene mutations.
BACKGROUND: HPV genotypes are the most common etiological factor for genital neoplasia. It would appear that sexually transmitted infections accompanied with HPV genotypes might have synergistic interactions in cancer progression. The genetic polymorphisms are involved in metabolizing carcinogens which may contribute to the susceptibility of developing genital cancers by less efficient or overly down metabolic pathways and cell signaling. MTHFR polymorphisms are related to several metabolic disorders and human cancers. We investigated the contribution of MTHFR 1298 and MTHFR 677 polymorphisms as potential risk factors for outcomes with HPV genotypes and STIs in Iranian population. MATERIALS AND METHODS: As a case-control study, MTHFR A1298C and C677T were assessed for SNPs analysis using a PCR-RFLP assay in 50 cervical intraepithelial neoplasia (CIN) cases, 98 HPV-positive subjects and 47 non-cancerous/non-HPV patients as healthy controls. RESULTS: Finding suggested a significant association between the MTHFR 1298 CC polymorphisms (OR = 3.5, 95% CI = 1.13-10.82, P ≤ 0.05) in women with CIN as compared to non-cancerous/non-HPV subjects. There was not a significant difference of MTHFR 677 between outcomes. DISCUSSION: It would seem MTHFR 1298 CC is more likely to be a potential risk factor for HPV-cervical cancer progression. Consequences support further attempts to understand the clinical manifestations of neoplasia related to genital infections and gene mutations.
Authors: M T Goodman; K McDuffie; B Hernandez; L R Wilkens; C C Bertram; J Killeen; L Le Marchand; J Selhub; S Murphy; T A Donlon Journal: Cancer Epidemiol Biomarkers Prev Date: 2001-12 Impact factor: 4.254
Authors: Amir Sohrabi; Siamak Mirab-Samiee; Mohammad Hossein Modarresi; Narges Izadimood; Kayhan Azadmanesh; Marjan Rahnamaye-Farzami Journal: Asian Pac J Cancer Prev Date: 2014
Authors: Amir Sohrabi; Siamak Mirab-Samiee; Marjan Rahnamaye-Farzami; Mitra Rafizadeh; Setareh Akhavan; Mohammad Hashemi-Bahremani; Mohammad Hossein Modarresl Journal: Asian Pac J Cancer Prev Date: 2014