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Literature DB >> 3340547

Identification of sequence-specific DNA-binding factors by label transfer: application to the adenovirus-2 major late promoter.

L G Nikolaev¹, B O Glotov, A V Belyavsky, S A Grachev, A V Levin.

Abstract

A method of affinity labelling proteins specifically associated with DNA target sequences is proposed. The method utilizes covalent UV-crosslinking of proteins to highly labelled DNA (e.g. in crude cell or nuclear extracts) followed by degradation of the DNA to short oligonucleotides. Proteins selectively labelled by attached residual oligonucleotides are readily amenable to molecular mass determination. Using this approach, we have characterized a HeLa polypeptide specifically bound to a short segment of the adenovirus-2 major late promoter (Ad2 MLP). A molecular mass value (approximately 51 kD) and precise location of the crosslinking site(s) of the protein within the MLP (-55 with respect to the cap site) were determined.

Entities: Chemical Species

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Year: 1988 PMID： 3340547 PMCID： PMC334676 DOI： 10.1093/nar/16.2.519

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

23 in total

Identification of sequence-specific DNA-binding factors by label transfer: application to the adenovirus-2 major late promoter.

1. Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.

2. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

3. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

4. Extensive regions of homology in front of the two hsp70 heat shock variant genes in Drosophila melanogaster.

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Review 6. Transcriptional control regions: nucleotide sequence requirements for initiation by RNA polymerase II and III.

7. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

8. Biogenesis, structures, and sites of encoding of the 5' termini of adenovirus-2 mRNAs.

9. The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.

10. DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.

1. Identification of a sequence-specific protein binding the 5'-transcribed spacer of rat ribosomal genes.

2. Analysis by cell-free transcription of the liver-specific pyruvate kinase gene promoter.