Literature DB >> 33405034

Fungal Bioreporters to Monitor Outcomes of Aspergillus: Host-Cell Interactions.

Neta Shlezinger1,2, Jeffrey Scott Fites3,4, Bruce S Klein5,6,7, Tobias M Hohl1,8.   

Abstract

Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host-fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the filamentous mold Aspergillus fumigatus.

Entities:  

Keywords:  Fluorescent reporters; Host-Aspergillus interactions

Mesh：

Substances：

Year:  2021        PMID： 33405034      PMCID： PMC9088164          DOI： 10.1007/978-1-0716-1182-1_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

Review 1.  Aspergillosis.

Authors:  Brahm H Segal
Journal:  N Engl J Med       Date:  2009-04-30       Impact factor: 91.245

2.  Role of Granulocyte-Macrophage Colony-Stimulating Factor Signaling in Regulating Neutrophil Antifungal Activity and the Oxidative Burst During Respiratory Fungal Challenge.

Authors:  Shinji Kasahara; Anupam Jhingran; Sourabh Dhingra; Anand Salem; Robert A Cramer; Tobias M Hohl
Journal:  J Infect Dis       Date:  2016-02-09       Impact factor: 5.226

3.  Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung.

Authors:  Alexander V Misharin; Luisa Morales-Nebreda; Gökhan M Mutlu; G R Scott Budinger; Harris Perlman
Journal:  Am J Respir Cell Mol Biol       Date:  2013-10       Impact factor: 6.914

4.  A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.

Authors:  Rajendra Upadhya; Woei C Lam; Brian T Maybruck; Maureen J Donlin; Andrew L Chang; Sarah Kayode; Kate L Ormerod; James A Fraser; Tamara L Doering; Jennifer K Lodge
Journal:  Fungal Genet Biol       Date:  2017-09-12       Impact factor: 3.495

5.  Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection.

Authors:  Heather L Clark; Anupam Jhingran; Yan Sun; Chairut Vareechon; Steven de Jesus Carrion; Eric P Skaar; Walter J Chazin; José Antonio Calera; Tobias M Hohl; Eric Pearlman
Journal:  J Immunol       Date:  2015-11-18       Impact factor: 5.422

6.  Fungal Mimicry of a Mammalian Aminopeptidase Disables Innate Immunity and Promotes Pathogenicity.

Authors:  Alana K Sterkel; Jenna L Lorenzini; J Scott Fites; Kavitha Subramanian Vignesh; Thomas D Sullivan; Marcel Wuthrich; Tristan Brandhorst; Nydiaris Hernandez-Santos; George S Deepe; Bruce S Klein
Journal:  Cell Host Microbe       Date:  2016-02-25       Impact factor: 31.316

7.  Myeloid derived hypoxia inducible factor 1-alpha is required for protection against pulmonary Aspergillus fumigatus infection.

Authors:  Kelly M Shepardson; Anupam Jhingran; Alayna Caffrey; Joshua J Obar; Benjamin T Suratt; Brent L Berwin; Tobias M Hohl; Robert A Cramer
Journal:  PLoS Pathog       Date:  2014-09-25       Impact factor: 6.823

8.  Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus.

Authors:  Shan F Brunel; Jude M Bain; Jill King; Lena J Heung; Shinji Kasahara; Tobias M Hohl; Adilia Warris
Journal:  J Vis Exp       Date:  2017-04-19       Impact factor: 1.355

Review 9.  Deploying FLAREs to Visualize Functional Outcomes of Host-Pathogen Encounters.

Authors:  Lena J Heung; Anupam Jhingran; Tobias M Hohl
Journal:  PLoS Pathog       Date:  2015-07-09       Impact factor: 6.823

10.  Inflammatory monocytes orchestrate innate antifungal immunity in the lung.

Authors:  Vanessa Espinosa; Anupam Jhingran; Orchi Dutta; Shinji Kasahara; Robert Donnelly; Peicheng Du; Jeffrey Rosenfeld; Ingrid Leiner; Chiann-Chyi Chen; Yacov Ron; Tobias M Hohl; Amariliz Rivera
Journal:  PLoS Pathog       Date:  2014-02-20       Impact factor: 6.823
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