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Literature DB >> 33405033

Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host-Cell Interactions.

Jeffrey Scott Fites^1,2, Neta Shlezinger^3,4, Tobias M Hohl^5,6, Bruce S Klein^1,7,8.

Abstract

Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host-fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.

Entities: Chemical

Keywords: Fluorescent reporters; Host-Blastomyces interactions

Mesh：

Substances：

Year: 2021 PMID： 33405033 DOI： 10.1007/978-1-0716-1182-1_7

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

8 in total

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3. Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection.

Authors: Nydiaris Hernández-Santos; Darin L Wiesner; J Scott Fites; Andrew J McDermott; Thomas Warner; Marcel Wüthrich; Bruce S Klein
Journal: Cell Host Microbe Date: 2018-03-22 Impact factor: 21.023

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5. Fungal Mimicry of a Mammalian Aminopeptidase Disables Innate Immunity and Promotes Pathogenicity.

Authors: Alana K Sterkel; Jenna L Lorenzini; J Scott Fites; Kavitha Subramanian Vignesh; Thomas D Sullivan; Marcel Wuthrich; Tristan Brandhorst; Nydiaris Hernandez-Santos; George S Deepe; Bruce S Klein
Journal: Cell Host Microbe Date: 2016-02-25 Impact factor: 31.316

Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host-Cell Interactions.

1. Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung.

2. A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.

3. Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection.

4. Tracing conidial fate and measuring host cell antifungal activity using a reporter of microbial viability in the lung.

5. Fungal Mimicry of a Mammalian Aminopeptidase Disables Innate Immunity and Promotes Pathogenicity.

Review 6. Clinical Manifestations and Treatment of Blastomycosis.

7. Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination.

Review 8. Deploying FLAREs to Visualize Functional Outcomes of Host-Pathogen Encounters.