Rongtao Liu1,2, Shiyang Zhang1,2, Chen Zhao1, Dong Yang1, Tingting Cui1, Yidong Liu3, Yonggang Min4,5. 1. School of Materials and Energy, Guangdong University of Technology (GDUT), Guangzhou, 510006, China. 2. Dongguan South China Design Innovation Institute, Dongguan, 523808, Guangdong, China. 3. School of Materials and Energy, Guangdong University of Technology (GDUT), Guangzhou, 510006, China. zlla@foxmail.com. 4. School of Materials and Energy, Guangdong University of Technology (GDUT), Guangzhou, 510006, China. ygmin@gdut.edu.cn. 5. Dongguan South China Design Innovation Institute, Dongguan, 523808, Guangdong, China. ygmin@gdut.edu.cn.
Abstract
Conductive and degradable nanofibrous scaffolds have great potential in promoting cell growth, proliferation, and differentiation under an external electric field. Although the issue of inferior electrical conductivity in body fluids still exists, polyaniline (PANI)-based degradable nanofibers can promote cell adhesion, growth, and proliferation. To investigate whether the effect is caused by the PANI morphology, we selected three inorganic acids as dopants in the process of PANI in situ oxidative polymerization: hydrochloric acid, sulfuric acid, and perchloric acid. The obtained polyaniline/polylactic acid (PANI/PLA) composite nanofibers were characterized via SEM, FTIR, and XPS analysis, and we confirmed that the PLA nanofibers were successfully coated by PANI without any change to the porous structure of the PLA nanofibers. The in vitro mechanical properties and degradability indicated that the oxidation of acid dopants should be considered and that it was likely to have a higher oxidation degradation effect on PLA nanofibers. The contact angle test demonstrated that PANI/PLA composite nanofibers with different surface morphologies have good wettability, implying that they meet the requirements of bone tissue engineering scaffolds. The surface roughness and cell viability demonstrated that different PANI morphologies on the surface can promote cell proliferation. The higher the surface roughness of the PANI, the better the biocompatibility. Consequently, the regulated surface morphology of PANI/PLA composite nanofibers via different acids doping has positive effect on biocompatibility in tissue engineering.
Conductive and degradable nanofibrous scaffolds have great potential in promoting cell growth, prolian class="Chemical">pan class="Gene">fean>ration, and difpaan>n class="Gene">ferentiation under an external electric field. Although the issue of inferior electrical conductivity in body fluids still exists, polyaniline (PANI)-based degradable nanofibers can promote cell adhesion, growth, and proliferation. To investigate whether the effect is caused by the PANI morphology, we selected three inorganic acids as dopants in the process of PANI in situ oxidative polymerization: hydrochloric acid, sulfuric acid, and perchloric acid. The obtained polyaniline/polylactic acid (PANI/PLA) composite nanofibers were characterized via SEM, FTIR, and XPS analysis, and we confirmed that the PLA nanofibers were successfully coated by PANI without any change to the porous structure of the PLA nanofibers. The in vitro mechanical properties and degradability indicated that the oxidation of acid dopants should be considered and that it was likely to have a higher oxidation degradation effect on PLA nanofibers. The contact angle test demonstrated that PANI/PLA composite nanofibers with different surface morphologies have good wettability, implying that they meet the requirements of bone tissue engineering scaffolds. The surface roughness and cell viability demonstrated that different PANI morphologies on the surface can promote cell proliferation. The higher the surface roughness of the PANI, the better the biocompatibility. Consequently, the regulated surface morphology of PANI/PLA composite nanofibers via different acids doping has positive effect on biocompatibility in tissue engineering.
Authors: Maradhana Agung Marsudi; Ridhola Tri Ariski; Arie Wibowo; Glen Cooper; Anggraini Barlian; Riska Rachmantyo; Paulo J D S Bartolo Journal: Int J Mol Sci Date: 2021-10-26 Impact factor: 5.923