An evaluation of six-year Stenotrophomonas maltophilia infections in a university hospital.

BACKGROUND: Stenotrophomonas maltophilia is a Gram-negative bacillus and opportunistic emergent pathogen causing hospital-acquired infections (HAIs). Due to risk factors such as prolonged intensive care unit stay and invasive procedures, it has become one of the leading causes of HAIs.
OBJECTIVE: The aim of this study was to evaluate the epidemiology of S.maltophilia infections over a six-year period at Düzce University Hospital, Turkey.
METHODS: The incidence, clinical characteristics, antimicrobial susceptibility and outcomes of nosocomial S. maltophilia infections during this period were retrospectively analyzed.
RESULTS: During the study period, 67 samples obtained from 61 patients were identified. Pneumonias (82%) were the most common HAIs, followed by bloodstream infections (10.5%), urinary tract infections (3%), skin and soft tissue infections (3%) and surgical site infection (1.5%). Admission to intensive care, hospitalization exceeding 30 days, and previous use of broad-spectrum antibiotics constituted risk factors. Resistance to cotrimoxazole (6%) was lower than that to levofloxacin (18%).
CONCLUSION: The most important risk factors for S.maltophilia infection in patients are previous exposure to antibiotics, prolonged hospitalization and invasive procedures such as mechanic ventilation. Discharging patients as early as possible with the rational use of antibiotics may be effective in reducing S. maltophilia infections and resistance rates.

Introduction

Stenotrophomonas maltophilia is an aerobic, non-fermentative, motile, sporeless, gram-negative bacillus widely present in nature1. It is currently the only species in the genus Stenotrophomonas2. The agent can be present in oropharyngeal and airway flora, and can be isolated from several environments in which humans live. It was previously thought to be a pathogen only in immunosuppressive diseases, but is now included among the agents implicated in nosocomial infections due to such risk factors as extended hospitalization, intensive care stay, and invasive procedures, even in immunocompetent patients1,2. Although cases have been reported in community-acquired infections, it is frequently isolated from infections developing in hospital3. Patients with a high risk of S.maltophilia infection include subjects with a previous hıstory of antibiotic therapy, chronic liver or kidney disease, connective tissue disorders, immune suppression due to HIV-positivity or malignancy, who are attached to mechanical ventilators or under follow-up in intensive care units, or with severe underlying comorbid disease4,5. Difficulties may be encountered in the treatment of S.maltophilia, which is naturally resistant to several antibiotics, such as penicillins, cephalosporins, carbapenems, and aminoglycosides6. Bacterial resistance to drugs develops for reasons such as biofilm formation, synthesis of drug-neutralizing enzymes, synthesis of false targets impervious to the drug, or alteration of permeability against the drug7,8. An increase in resistance rates has been observed to antibiotics such as cotrimoxazole and levofloxacin9.

The epidemiology of nosocomial infections with S.maltophilia varies greatly, depending on the healthcare institution profile and geographical location. Evaluation of local data is therefore essential in order to assess trends over time and to describe the national situation compared to international data. The purpose of this retrospective study was to examine the clinical characteristics, underlying risk factors, and antibiotic resistance rates of nosocomial S.maltophilia infections in 2013–2018, and to compare the results with other findings in the literature.

Methods

This retrospective study was conducted at the Duzce University Education and Research Hospital, located in the northwest of Turkey and serving as a tertiary care referral hospital, from January 2013 to December 2018. All hospitalized patients aged 18 years or above with nosocomial infections caused by S.maltophilia were included in the study. Patients' medical records of patients, including clinical microbiology and Hospital Infection Control Committee reports, were evaluated, and demographic features, clinical conditions, laboratory data, antimicrobial susceptibility, and outcomes were analyzed retrospectively. Ethics committee approval for our study was obtained from the Düzce University Faculty of Medicine Ethics Committee (No. 2019/127). Nosocomial infections were diagnosed based on Centers for Disease Control and Prevention (CDC) recommendations10. Since nosocomial S.maltophilia infection developed twice at different times in six of the 61 patients, the sample number was calculated as 67. The patient number (n:61) was therefore used in calculations involving patient age, sex, unit of admission, risk factors, and mortality rates (n:61), while S.maltophilia numbers (n:67) were used in calculations such as specimen type, infection diagnosis, and antibiotic sensitivity.

Laboratory methods

Clinical samples sent to the microbiology laboratory under appropriate conditions were added to 5% sheep blood agar and Eosin Methylene Blue (EMB) (Oxoid, UK) agar and incubated in an aerobic environment for 24 h at 35–37°C. Blood specimens placed into blood culture tubes under sterile conditions for culture were incubated without delay in a BACTEC blood culture device. Five percent sheep blood agar and EMB agar was added to tubes with positive growth. Conventional methods and a Vitek-2 automated system (BioMérieux, France) were used to identify the growing bacteria and to determine their antbiotic sensitivities. Bacterial antibiotic sensitivities were determined in the light of Clinical Laboratory Standards Institute (CLSI) recommendations in 2013–2014, and 2015–2018 European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations in 2015–2018.

Statistical analysis

Statistical analysis was performed on SPSS for Windows 14.0 software. The descriptive values of the data obtained were expressed as mean, standard deviation, number, and percentage frequencies.

Results

During the six-year study period, 67 samples from 61 patients were identified as culture-positive for S.maltophilia. Sixty-six percent (n:40) of the patients were men, and the patients' mean age was 64.34±19.24 years (18–93). Mean length of hospitalization was 75.01±58.02 days, with 82% (n:50) of patients admitted to the intensive care unit and 18% (n:11) admitted to the wards. In terms of admission diagnoses at initial hospitalization, 33% (n:22) were admitted with a preliminary diagnosis of infection. Eighty-two percent (n:55) of the nosocomial infections developing with S.maltophilia consisted of pneumonia (n:55). Other diagnoses included bacteremia (10.5%), urinary tract infection (3%), cutaneous and soft tissue infections (3%) and surgical site infections (1.5%). Use of mechanical ventilation was present as an invasive procedure in two-thirds of patients (72%). The other most commonly identified risk factors were admission to intensive care (82%), hospital stay exceeding 30 days (74%, and previous use of broad-spectrum antibiotics (64%). The general mortality rate in all patients with S.maltophilia growth was 49% (n:30). Demographic data for patients with nosocomial infection and patient characteristics are shown in Table 1.

Table 1

Demographic and basic characteristics of the 61 patients infected with S. maltophilia

Patient characteristics	n (%)
Age (mean)	64.34±19.24 (18–93)
Sex
Male/Female	40/21
Days of hospitalization (mean±SD)	75.01±58.02
Admission Diagnosis
Infectious diseases	22 (33)
Cerebrovascular disease	13 (19.5)
Respiratory insufficiency	8 (12)
Trauma	7 (10.5)
Cardiac arrest	6 (9)
Other	11 (16)
Comorbid Diseases
Hypertension	28 (61)
DM	16 (26)
Heart failure	12 (20)
Cerebrovascular disease	10 (16)
COPD	8 (13)
Malignancy	7 (11)
Chronic kidney failure	4 (7)
Other	28 (46)
Nosocomial Infection Diagnosis
Pneumonia	55 (82)
Bacteremia	7 (10.5)
Urinary tract infection	2 (3)
Skin and soft tissue infection	2 (3)
Surgical site infection	1 (1.5)
Devices Used
Invasive mechanical ventilation	44 (72)
Central venous catheter	28 (46)
Urinary catheter	58 (95)
Other risk factors
Follow-up in intensive care	50 (82)
Surgical intervention	14 (23)
Broad-spectrum antibiotic use	39 (64)
Hospitalization exceeding 30 days	45 (74)
Mortality	30 (49)

Analysis of isolated strains by years revealed an increase in 2015–2017, but a decrease in 2018. Tracheal aspirate constituted 75% (n:50) of the specimens collected. The others were collected from phlegm in four, catheter in four, blood in three, urine in two, and bronchoalveolar lavage in one. Forty seven of the 67 S.maltophilia infected patients had co-infection. The most common agent in polymicrobial samples was Acinetobacter baumannii. All 67 isolates were tested for antimicrobial resistance, and the most susceptible antibiotics were levofloxacin and cotrimoxazole (82% and 94% susceptibility, respectively). No strains were simultaneously resistant to both antibiotics. Distributions of samples by years and specimen type antibiotic sensitivities are shown in Table 2.

Table 2

Strain distributions by years, specimen characteristics, and antibiotic susceptibilities

Sample characteristics	n (percentage)
Year
2013	5 (7.5)
2014	5 (7.5)
2015	20 (30)
2016	19 (28.5)
2017	13 (19)
2018	5 (7.5)
Specimen type
Deep tracheal aspirate	50 (75)
Phlegm	4 (6)
Catheter	4 (6)
Blood	3 (4)
Wound	3 (4)
Urine	2 (3)
Bronchoalveolar lavage	1 (1.5)
Antibiotic susceptibility
Levofloxacin	55 (82)
Cotrimoxazole	63 (94)

Discussion

Increasing numbers of nosocomial infections developing due to S.maltophilia are being reported for reasons such as prolonged hospitalization, the use of broad-spectrum antibiotics, and an increase in the numbers of immunosuppressive patients11. The most common infections developing with this bacterium are pneumonia and bloodstream infections, while wound and urinary tract infections are less common12. The current retrospective study aimed to identify the differences of demographic and clinical characteristics, microbiological findings and the final mortality outcomes of patients with nosocomial infection caused by S. maltophilia.

S.maltophilia is a microorganism that can be found in the hospital environment. It causes colonization in various medical devices, leading to nosocomial infection11. High rates of polymicrobial infection have also been reported in these patients13. A high rate of polymicrobial growth (70%) was also determined in the present study. The most common agent in polymicrobial specimens was Acinetobacter spp. Candevir Ulu et al. also identified Acinetobacter spp. as the most frequently seen agent (40.1%)14. We attribute this to low hand hygiene compliance in intensive care units at that time. Studies in which S.maltophilia has particularly been isolated in airway and blood specimens have also determined this most commonly in airway specimens (75%).

Due to the greater prevalence of various predisposing factors, patients admitted to intensive care represent the majority of patients in studies involving S.maltophilia15. Intensive care patients constituted 82% (n:50) of the patients in our study. Other risk factors for S.maltophilia in our study were hospitalization exceeding 30 days (74%), use of broad-spectrum antibiotics (64%), and mechanical ventilation (72%). Previous studies have reported mortality rates of 12–69% for S.maltophilia infection4,8,15. In agreement with the previous literature, the mortality rate in our study was 49%. The inconsistent findings may be due to variations in patient populations or in other factors contributing to mortality. A history of use of broad-spectrum antibiotics prior to infections developing with S.maltophilia has been reported as a risk factor in several previous studies4. Previous treatment with antipseudomonal broad-spectrum antibiotics has been reported as a risk factor for S.maltophilia bacteremia in some studies16. In contrast, Sumida et al. reported that previous use of antibiotics effective against methicillin-resistant Staphylococcus aureus, but not carbapenems, represented a risk factor for S.maltophilia bacteremia17. The rate of previous antibiotic use in our study was 64%, and the fact that antibiotic groups were not differentiated is one of the limitations of this study. The fact that antibiotics such as carbapenem and cephalosporin that are frequently employed in empiric treatment are naturally resistant to S. maltophilia delays treatment until cultures are obtained and increases mortality18. One recent review study determined an increase in rates of S. maltophilia resistance to cotrimoxazole and levofloxacin. According to that review study, susceptibility dtermined in different countries was 80–99% for TMP-SXT and 44–97% for levofloxacin19. One study of two centers in Turkey at different times reported a time-dependent decrease in cotrimoxazole susceptibility20,21. The low cotrimoxazole resistance in our study is a welcome finding for our institution as a local datum. Since S. maltophilia is generally susceptible to quinolones, these have become an important empiric treatment option on non-fermentative bacterial infections22. Among susceptibility studies involving levofloxacin, Gozel et al. from Turkey determined a low level of S. maltophilia levofloxacin resistance, at 2.9%21. In contrast, Cho et al. reported that their high rate of resistance to levofloxacin, 56%, derived from their patient group consisting of individuals with hematological malignancy23. The levofloxacin resistance rate in our study was 18%, which is in agreement with the previous literature. The use of levofloxacin in our hospital may need to be evaluated to achieve further improvement in levofloxacin susceptibility.

Conclusion

There are a number of limitations to this study. The first is its retrospective nature. Other important limitations include the sample size, which might be insufficient for adequately assessing the prognostic factors in S. maltophilia-associated infection. Further epidemiological multi-center studies involving longer surveillance are therefore now needed for a better understanding of the prevalence and distribution of S. maltophilia-associated nosocomial infections.