DA-Tian Bau1,2, Wen-Shin Chang1, Guo-Kai Wang3,4, Jai-Sing Yang5, Yu-Fei Huang3, Jin-Song Liu3,4, Chia-Wen Tsai5. 1. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.; datian@mail.cmuh.org.tw artbau2@gmail.com. 2. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C. 3. School of Pharmacy, Anhui University of Chinese Medicine, Hefei, P.R. China. 4. Anhui Province Key Laboratory of Research and Development of Chinese Medicine, Hefei, P.R. China. 5. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND: Salvia miltiorrhiza is a medical herb for human disorders including cardiovascular diseases and cancer. However, the interactions between Salvia miltiorrhiza and its endophytes are largely unknown. The current study aimed at identifying its endophytic fungi and examining their inhibitory effects on anti-pathogenic fungus. MATERIALS AND METHODS: Distinct species of endophytic fungi were isolated from the roots of Salvia miltiorrhiza, cultured, sequenced, aiming to predict their taxonomical structures. Meanwhile, extracts from each endophytic fungus fermentations were isolated, compared and evaluated on the inhibitory efficacies on five pathological fungi, Cercospora nicotianae, Phoma arachnidicola, Staphylococcus, Phytophthora eggplant, and Rhizoctonia cerealis. RESULTS: A total of 34 strains of endophytic fungi were obtained from Salvia miltiorrhiza. Among them, SX19 and C. Gloeosporioids exhibited the most effective inhibitions on five pathogenic fungi. CONCLUSION: The anti-fungal activities of the endophytic fungus from Salvia miltiorrhiza were confirmed for the first time, and this may benefit crop quality and production in the future. Copyright
BACKGROUND:Salvia miltiorrhiza is a medical herb for human disorders including cardiovascular diseases and cancer. However, the interactions between Salvia miltiorrhiza and its endophytes are largely unknown. The current study aimed at identifying its endophytic fungi and examining their inhibitory effects on anti-pathogenic fungus. MATERIALS AND METHODS: Distinct species of endophytic fungi were isolated from the roots of Salvia miltiorrhiza, cultured, sequenced, aiming to predict their taxonomical structures. Meanwhile, extracts from each endophytic fungus fermentations were isolated, compared and evaluated on the inhibitory efficacies on five pathological fungi, Cercospora nicotianae, Phoma arachnidicola, Staphylococcus, Phytophthora eggplant, and Rhizoctonia cerealis. RESULTS: A total of 34 strains of endophytic fungi were obtained from Salvia miltiorrhiza. Among them, SX19 and C. Gloeosporioids exhibited the most effective inhibitions on five pathogenic fungi. CONCLUSION: The anti-fungal activities of the endophytic fungus from Salvia miltiorrhiza were confirmed for the first time, and this may benefit crop quality and production in the future. Copyright
Authors: Jian Sun; Shan Hong Huang; Benny K-H Tan; Matt Whiteman; Yi Chun Zhu; Ya Jun Wu; Yeekong Ng; Wei Duan; Yi Zhun Zhu Journal: Life Sci Date: 2005-04-29 Impact factor: 5.037