Satoshi Yokose1, Yuka Kato2, Katsutoshi Matsumoto2, Perry R Klokkevold3, Henry H Takei3, Hiroshi Kawazu4, Hiroshi Sakagami5. 1. Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan; s-yokose@dent.meikai.ac.jp. 2. Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan. 3. Division of Constitutive and Regenerative Sciences, Section of Periodontics, School of Dentistry, University California Los Angeles, Los Angeles, CA, U.S.A. 4. Section of Continuous Education, School of Dentistry, Meikai University, Saitama, Japan. 5. Meikai University Research Insititute of Odontology (M-RIO), Meikai University School of Dentistry, Saitama, Japan.
Abstract
BACKGROUND: Enamel matrix derivative (EMD) is widely used for regeneration therapy in dental clinical situations, but the mechanism of EMD bioactivity remains obscure. To clarify this mechanism, we focused on the formation of connective tissue and blood vessels. The aim of this study was to confirm whether EMD induces the formation of connective tissue and blood vessels by using the diffusion chamber (DC) technique. MATERIALS AND METHODS: Individual DCs containing EMD (DC-EMD) or propylene glycol alginate (PGA) were implanted subcutaneously in rat dorsum. At 4 weeks after the implantation, histological analysis of DCs was performed using azan staining. RESULTS: DC-EMD induced the formation of much larger amounts of connective tissue containing abundant blood vessels than did DC-PGA. CONCLUSION: The results indicated that EMD can induce the formation of both connective tissue and blood vessels. This bioactivity may contribute to the mechanism whereby EMD induces tissue regeneration. Copyright
BACKGROUND: Enamel matrix derivative (EMD) is widely used for regeneration therapy in dental clinical situations, but the mechanism of EMD bioactivity remains obscure. To clarify this mechanism, we focused on the formation of connective tissue and blood vessels. The aim of this study was to confirm whether EMD induces the formation of connective tissue and blood vessels by using the diffusion chamber (DC) technique. MATERIALS AND METHODS: Individual DCs containing EMD (DC-EMD) or propylene glycol alginate (PGA) were implanted subcutaneously in rat dorsum. At 4 weeks after the implantation, histological analysis of DCs was performed using azan staining. RESULTS: DC-EMD induced the formation of much larger amounts of connective tissue containing abundant blood vessels than did DC-PGA. CONCLUSION: The results indicated that EMD can induce the formation of both connective tissue and blood vessels. This bioactivity may contribute to the mechanism whereby EMD induces tissue regeneration. Copyright
Authors: Marco Esposito; Maria Gabriella Grusovin; Nikolaos Papanikolaou; Paul Coulthard; Helen V Worthington Journal: Cochrane Database Syst Rev Date: 2009-10-07
Authors: S Yokose; T Ishizuya; T Ikeda; T Nakamura; H Tsurukami; K Kawasaki; T Suda; S Yoshiki; A Yamaguchi Journal: Endocrinology Date: 1996-02 Impact factor: 4.736
Authors: Howard H Wang; Nima D Sarmast; Elham Shadmehr; Nikola Angelov; Shahrokh Shabahang; Mahmoud Torabinejad Journal: J Endod Date: 2018-03-24 Impact factor: 4.171