Wahyuni Lukita Atmodjo1,2, Young Othiwi Larasati3, Juandy Jo4, Riska Nufika3, Steffi Naomi5, Imelda Winoto3. 1. Department of Immunopathology, Mochtar Riady Institute for Nanotechnology, Tangerang, Indonesia; wahyuni.atmodjo@uph.edu. 2. Department of Anatomy, Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Indonesia. 3. Department of Immunopathology, Mochtar Riady Institute for Nanotechnology, Tangerang, Indonesia. 4. Program of Biology, Faculty of Science and Technology, Universitas Pelita Harapan, Tangerang, Indonesia. 5. Department of Biomedicine, Indonesia International Institute for Life Science, Jakarta, Indonesia.
Abstract
BACKGROUND/AIM: In vivo studies on pathogenesis of type 2 diabetes mellitus (T2DM) have been reported, however, the relationship between insulin-receptor substrate 1 (IRS1) and the area of Langerhans' islets was unknown. Therefore, a correlation between both parameters was assessed. MATERIALS AND METHODS: Diabetic groups were fed with a high-fat diet (HFD) and injected with three different doses of streptozotocin, namely 25, 35 and 45 mg/kg, and compared to a control group after 9 weeks. RESULTS: Administration of HFD/streptozotocin increased the level of fasting blood glucose but reduced the level of IRS1 and the area of Langerhans' islets in diabetic groups. The coefficient of correlation between IRS1 and area of Langerhans' islets was 0.259 (p=0.232). In addition, the coefficient of correlation for fasting blood glucose with the area of Langerhans' islets and IRS1 was -0.520 (p=0.011) and -0.603 (p=0.002), respectively. CONCLUSION: The reduction of IRS1 was weakly correlated with the destruction of Langerhans' islets, suggesting there is an intermediate step between both parameters. Copyright
BACKGROUND/AIM: In vivo studies on pathogenesis of type 2 diabetes mellitus (T2DM) have been reported, however, the relationship between insulin-receptor substrate 1 (IRS1) and the area of Langerhans' islets was unknown. Therefore, a correlation between both parameters was assessed. MATERIALS AND METHODS: Diabetic groups were fed with a high-fat diet (HFD) and injected with three different doses of streptozotocin, namely 25, 35 and 45 mg/kg, and compared to a control group after 9 weeks. RESULTS: Administration of HFD/streptozotocin increased the level of fasting blood glucose but reduced the level of IRS1 and the area of Langerhans' islets in diabetic groups. The coefficient of correlation between IRS1 and area of Langerhans' islets was 0.259 (p=0.232). In addition, the coefficient of correlation for fasting blood glucose with the area of Langerhans' islets and IRS1 was -0.520 (p=0.011) and -0.603 (p=0.002), respectively. CONCLUSION: The reduction of IRS1 was weakly correlated with the destruction of Langerhans' islets, suggesting there is an intermediate step between both parameters. Copyright
Authors: Anne M Flanagan; Jackie L Brown; Consuelo A Santiago; Pauline Y Aad; Leon J Spicer; Maria T Spicer Journal: J Nutr Biochem Date: 2007-09-27 Impact factor: 6.048