Charlene Herbemont1, Sonia Mnallah2, Badria Bennani-Smires1, Maeliss Peigne3, Isabelle Cedrin-Durnerin3, Michael Grynberg3, Christophe Sifer4. 1. Department of Reproductive Biology, Hôpitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France; University Paris XIII, Sorbonne Paris Cité, UFR SMBH, 93000, Bobigny, France. 2. Department of Reproductive Biology, Hôpitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France. 3. Department of Reproductive Medicine, Hopitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France. 4. Department of Reproductive Biology, Hôpitaux Universitaires Paris Seine Saint-Denis, AP-HP, 93140, Bondy, France; University Paris XIII, Sorbonne Paris Cité, UFR SMBH, 93000, Bobigny, France. Electronic address: christophe.sifer@aphp.fr.
Abstract
PURPOSE: The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS: A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers: a commercially-available, Cell Sleeper, and a "home-made" one, Stripper Tip. This man and his 30-year-old wife underwent an ICSI attempt using vitrified-warmed spermatozoa from these devices. All frozen-warmed spermatozoa were quickly recovered. Among the oocytes retrieved, six were injected with sperm from the Cell Sleeper, and seven with sperm from the Stripper tip, leading to 4 embryos in each case. Two embryos, arising from sperm frozen in the Stripper tip, were transferred, resulting in a healthy live-birth. CONCLUSIONS: This is the first successful delivery following the use of spermatozoa frozen in an original device, the Stripper Tip, giving a promising prospect for managing severe male infertilities.
PURPOSE: The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS: A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers: a commercially-available, Cell Sleeper, and a "home-made" one, Stripper Tip. This man and his 30-year-old wife underwent an ICSI attempt using vitrified-warmed spermatozoa from these devices. All frozen-warmed spermatozoa were quickly recovered. Among the oocytes retrieved, six were injected with sperm from the Cell Sleeper, and seven with sperm from the Stripper tip, leading to 4 embryos in each case. Two embryos, arising from sperm frozen in the Stripper tip, were transferred, resulting in a healthy live-birth. CONCLUSIONS: This is the first successful delivery following the use of spermatozoa frozen in an original device, the Stripper Tip, giving a promising prospect for managing severe male infertilities.