| Literature DB >> 33400387 |
Glenn A Marsh1, Alexander J McAuley1, Sheree Brown1, Elizabeth A Pharo1, Sandra Crameri1, Gough G Au1, Michelle L Baker1, Jennifer A Barr1, Jemma Bergfeld1, Matthew P Bruce1, Kathie Burkett1, Peter A Durr1, Clare Holmes1, Leonard Izzard1, Rachel Layton1, Suzanne Lowther1, Matthew J Neave1, Timothy Poole1, Sarah-Jane Riddell1, Brenton Rowe1, Elisha Soldani1, Vittoria Stevens1, Willy W Suen1, Vinod Sundaramoorthy1,2, Mary Tachedjian1, Shawn Todd1, Lee Trinidad1, Sinéad M Williams1, Julian D Druce3, Trevor W Drew1, Seshadri S Vasan1,4.
Abstract
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emerging virus that has caused significant human morbidity and mortality since its detection in late 2019. With the rapid emergence has come an unprecedented programme of vaccine development with at least 300 candidates under development. Ferrets have proven to be an appropriate animal model for testing safety and efficacy of SARS-CoV-2 vaccines due to quantifiable virus shedding in nasal washes and oral swabs. Here, we outline our efforts early in the SARS-CoV-2 outbreak to propagate and characterize an Australian isolate of the virus in vitro and in an ex vivo model of human airway epithelium, as well as to demonstrate the susceptibility of domestic ferrets (Mustela putorius furo) to SARS-CoV-2 infection following intranasal challenge.Entities:
Keywords: COVID-19; SARS-CoV-2, TEM; animal model; coronavirus; electron microscopy, ex vivo; ferret, in vitro, in vivo
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Year: 2021 PMID： 33400387 DOI： 10.1111/tbed.13978
Source DB: PubMed Journal: Transbound Emerg Dis ISSN： 1865-1674 Impact factor: 5.005