Literature DB >> 33398191

DeepCell Kiosk: scaling deep learning-enabled cellular image analysis with Kubernetes.

Dylan Bannon1, Erick Moen2, Morgan Schwartz2, Enrico Borba1, Takamasa Kudo3, Noah Greenwald4, Vibha Vijayakumar1, Brian Chang2, Edward Pao2, Erik Osterman5, William Graf2, David Van Valen6.   

Abstract

Deep learning is transforming the analysis of biological images, but applying these models to large datasets remains challenging. Here we describe the DeepCell Kiosk, cloud-native software that dynamically scales deep learning workflows to accommodate large imaging datasets. To demonstrate the scalability and affordability of this software, we identified cell nuclei in 106 1-megapixel images in ~5.5 h for ~US$250, with a cost below US$100 achievable depending on cluster configuration. The DeepCell Kiosk can be downloaded at https://github.com/vanvalenlab/kiosk-console ; a persistent deployment is available at https://deepcell.org/ .

Entities:  

Mesh:

Year:  2021        PMID: 33398191      PMCID: PMC8759612          DOI: 10.1038/s41592-020-01023-0

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   47.990


  17 in total

1.  TNIK Inhibition Has Dual Synergistic Effects on Tumor and Associated Immune Cells.

Authors:  Jaehee Kim; Juhyun Oh; Hannah M Peterson; Jonathan C T Carlson; Mikael J Pittet; Ralph Weissleder
Journal:  Adv Biol (Weinh)       Date:  2022-06-08

2.  Integrated Analytical System for Clinical Single-Cell Analysis.

Authors:  Hannah M Peterson; Lip Ket Chin; Yoshi Iwamoto; Juhyun Oh; Jonathan C T Carlson; Hakho Lee; Hyungsoon Im; Ralph Weissleder
Journal:  Adv Sci (Weinh)       Date:  2022-05-04       Impact factor: 17.521

3.  DeepBacs for multi-task bacterial image analysis using open-source deep learning approaches.

Authors:  Christoph Spahn; Estibaliz Gómez-de-Mariscal; Romain F Laine; Pedro M Pereira; Lucas von Chamier; Mia Conduit; Mariana G Pinho; Guillaume Jacquemet; Séamus Holden; Mike Heilemann; Ricardo Henriques
Journal:  Commun Biol       Date:  2022-07-09

Review 4.  Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging.

Authors:  John W Hickey; Elizabeth K Neumann; Andrea J Radtke; Jeannie M Camarillo; Rebecca T Beuschel; Alexandre Albanese; Elizabeth McDonough; Julia Hatler; Anne E Wiblin; Jeremy Fisher; Josh Croteau; Eliza C Small; Anup Sood; Richard M Caprioli; R Michael Angelo; Garry P Nolan; Kwanghun Chung; Stephen M Hewitt; Ronald N Germain; Jeffrey M Spraggins; Emma Lundberg; Michael P Snyder; Neil L Kelleher; Sinem K Saka
Journal:  Nat Methods       Date:  2021-11-22       Impact factor: 47.990

5.  Omnipose: a high-precision morphology-independent solution for bacterial cell segmentation.

Authors:  Kevin J Cutler; Carsen Stringer; Teresa W Lo; Luca Rappez; Nicholas Stroustrup; S Brook Peterson; Paul A Wiggins; Joseph D Mougous
Journal:  Nat Methods       Date:  2022-10-17       Impact factor: 47.990

Review 6.  Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.

Authors:  Mojca Mattiazzi Usaj; Clarence Hue Lok Yeung; Helena Friesen; Charles Boone; Brenda J Andrews
Journal:  Cell Syst       Date:  2021-06-16       Impact factor: 11.091

7.  Avoiding a replication crisis in deep-learning-based bioimage analysis.

Authors:  Romain F Laine; Ignacio Arganda-Carreras; Ricardo Henriques; Guillaume Jacquemet
Journal:  Nat Methods       Date:  2021-10       Impact factor: 28.547

8.  Democratising deep learning for microscopy with ZeroCostDL4Mic.

Authors:  Lucas von Chamier; Romain F Laine; Johanna Jukkala; Christoph Spahn; Daniel Krentzel; Elias Nehme; Martina Lerche; Sara Hernández-Pérez; Pieta K Mattila; Eleni Karinou; Séamus Holden; Ahmet Can Solak; Alexander Krull; Tim-Oliver Buchholz; Martin L Jones; Loïc A Royer; Christophe Leterrier; Yoav Shechtman; Florian Jug; Mike Heilemann; Guillaume Jacquemet; Ricardo Henriques
Journal:  Nat Commun       Date:  2021-04-15       Impact factor: 14.919

Review 9.  Data science in cell imaging.

Authors:  Meghan K Driscoll; Assaf Zaritsky
Journal:  J Cell Sci       Date:  2021-04-01       Impact factor: 5.285

10.  CellSeg: a robust, pre-trained nucleus segmentation and pixel quantification software for highly multiplexed fluorescence images.

Authors:  Michael Y Lee; Jacob S Bedia; Salil S Bhate; Graham L Barlow; Darci Phillips; Wendy J Fantl; Garry P Nolan; Christian M Schürch
Journal:  BMC Bioinformatics       Date:  2022-01-18       Impact factor: 3.169

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