| Literature DB >> 3339575 |
K M Ebert1, H Liem, N B Hecht.
Abstract
Total DNA was extracted from mouse embryos that were collected from CD-1 random-bred females on Day 1 of pregnancy and cultured for up to 4 days in vitro, or from the reproductive tracts of pregnant females on Days 1, 3, 4 and 5 of pregnancy. Southern blot analyses with a cloned mouse mitochondrial DNA probe were performed to determine the relative levels of mitochondrial DNA in the zygote, morula, blastocyst and early egg cylinder stage embryos. The results indicated that the total amount of mitochondrial DNA does not change during development of the mouse embryo up to the egg cylinder stage and is not altered during in-vitro culture of the fertilized one-cell embryo to the blastocyst stage.Entities:
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Year: 1988 PMID： 3339575 DOI： 10.1530/jrf.0.0820145
Source DB: PubMed Journal: J Reprod Fertil ISSN： 0022-4251