Literature DB >> 3339254

Sandwich-type ELISA for free and bound secretory component in human biological fluids.

C Vincent1, J P Revillard.   

Abstract

Three sandwich-type enzyme-linked immunosorbent assays (ELISA) are described for the measurement of free secretory component (SC) and SC bound to IgA (S-IgA) or IgM (S-IgM). These assays do not require preliminary fractionation of the biological fluids to be tested. The specificity of the assays is achieved with monoclonal antibodies specific for free SC (855 SC) and for SC bound to IgA or IgM (8545 SA). The amount of the three SC molecules in various biological fluids is reported. We demonstrate the presence of low levels of free SC in most of these fluids, including normal serum. Moreover our results suggest that S-IgM in serum may result from a non-covalent association between serum IgM and free SC.

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Year:  1988        PMID： 3339254     DOI： 10.1016/0022-1759(88)90191-3

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Secretory IgA are elevated in both saliva and serum of patients with various types of primary glomerulonephritis.

Authors:  G Rostoker; H Terzidis; M Petit-Phar; D Meillet; P Lang; J M Dubert; G Lagrue; B Weil
Journal:  Clin Exp Immunol       Date:  1992-11       Impact factor: 4.330

2.  Lactoferrin and secretory IgA in the bronchoalveolar lavage fluid from patients with a stable asthma.

Authors:  E A van de Graaf; T A Out; A Kobesen; H M Jansen
Journal:  Lung       Date:  1991       Impact factor: 2.584

3.  Serum secretory immunoglobulins in ankylosing spondylitis.

Authors:  D Wendling; J M Didier; E Seilles
Journal:  Clin Rheumatol       Date:  1996-11       Impact factor: 2.980

4.  Spontaneous expression of a low affinity Fc receptor for IgA (Fc alpha R) on human B cell lines.

Authors:  I Millet; F Briere; C Vincent; F Rousset; C Andreoni; J E De Vries; J P Revillard
Journal:  Clin Exp Immunol       Date:  1989-05       Impact factor: 4.330

5.  Inhibition of human peripheral blood mononuclear cell proliferative response by glycosphingolipids from metacestodes of Echinococcus multilocularis.

Authors:  F Persat; C Vincent; D Schmitt; M Mojon
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

6.  Secretory immunoglobulins in serum from human immunodeficiency virus (HIV)-infected patients.

Authors:  C Vincent; G Cozon; M Zittoun; M Mellquist; M D Kazatchkine; C Czerkinsky; J P Revillard
Journal:  J Clin Immunol       Date:  1992-09       Impact factor: 8.317

7.  Reduced inhibition of Candida albicans adhesion by saliva from patients receiving oral cancer therapy.

Authors:  M Umazume; E Ueta; T Osaki
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

8.  Alterations in polymeric immunoglobulin receptor expression and secretory component levels in bladder carcinoma.

Authors:  M Rossel; C Billerey; H Bittard; P Ksiazek; D Alber; J P Revillard; D A Vuitton
Journal:  Urol Res       Date:  1991

9.  CD14 and CD11b mediate serum-independent binding to human monocytes of an acylpolygalactoside isolated from Klebsiella pneumoniae.

Authors:  Z Hmama; A Mey; G Normier; H Binz; J P Revillard
Journal:  Infect Immun       Date:  1994-05       Impact factor: 3.441

10.  Is there IgA of gut mucosal origin in the serum of HIV1 infected patients?

Authors:  A Quesnel; P Moja; F Lucht; J L Touraine; B Pozzetto; C Genin
Journal:  Gut       Date:  1994-06       Impact factor: 23.059
  10 in total

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