Fasciola hepatica: perturbation of secretory activity in the vitelline cells by the sodium ionophore monensin.

The effect of the sodium ionophore monensin on the vitelline cells of Fasciola hepatica has been determined both in vitro and in vivo by means of transmission electron microscopy. In intact flukes in vitro, vacuolation of the Golgi complex of the intermediate, shell protein secreting vitelline cells is evident after 1.5 hr incubation in monensin (1 X 10(-6) M). The vacuolation becomes progressively greater with time, eventually spreading to the late stem cells and mature cells. In addition, there is a block in the normal migration of the shell protein globules to the periphery, the shell globule clusters becoming very loosely packed and empty, and distended single globules accumulate in the perinuclear region of the cell. Disruption of the nurse cell cytoplasm is apparent from 6 hr onwards, giving the follicle a less compact appearance. Morphological changes induced by higher concentrations of monensin (up to 1 X 10(-4) M) followed a similar time course and pattern to that described for 1 X 10(-6) M) followed a similar time course and pattern to that described for 1 X 10(-6) M. In tissue-slice material (1 X 10(-6) M) these effects of monensin are evident more rapidly, and to a far greater extent: the condition of the vitelline cells in slices after only 1.5 hr resembles that reached in intact flukes after more than a 12-hr incubation. Incubation in ouabain, an inhibitor of Na+/K+-ATPase activity, has little effect on vitelline morphology over a 6-hr period (1 X 10(-3) M), although brief (0.5 hr) exposure to ouabain followed by monensin treatment (1 X 10(-4) M, 3 hr) does lead to gross vacuolation of the intermediate cells, the condition resembling that in tissue-slice material. In contrast, in vivo treatment of infected laboratory rats (1 X 5 mg/kg) only leads to a transient effect on the ultrastructure of the intermediate vitelline and nurse cells. The specific perturbation of the Golgi complex and secretory traffic in the vitelline cells of F. hepatica by monensin follows the classic pattern observed in other cell types.