Danshan Gu1, Haoan Yi2, Kerong Jiang1, Syed Hassam Fakhar3, Jing Shi1, Yongshu He2, Bo Liu4, Yunping Guo5, Xiaoming Fan6, Shude Li7. 1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Kunming medical university, Kunming, Yunnan 650500, PR China. 2. Department of Cell Biology and Medical Genetics, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China. 3. School of Public Health, Kunming Medical University, Kunming, Yunnan 650500, PR China. 4. School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming medical university, Kunming, Yunnan 650500, PR China. 5. School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming medical university, Kunming, Yunnan 650500, PR China. Electronic address: Pigeon5073@163.com. 6. Department of Anthropotomy, School of Basic Medicine, Guilin Medical University, Guilin, Guangxi 541004, PR China. Electronic address: fanxiaom1987@glmc.edu.cn. 7. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Kunming medical university, Kunming, Yunnan 650500, PR China. Electronic address: Shudeli006@163.com.
Abstract
AIMS: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease and lacks for safe and effective drug to therapy completely. Ginsenoside-Rg1 is one of the main components of ginseng and has been proved to counteract a variety of diseases. However, there is currently a lack of sufficient evidence to support the efficacy of ginsenoside-Rg1 in the treatment of NAFLD. Our aim was to investigate whether Ginsenoside-Rg1 is a potential drug for NAFLD. MAIN METHODS: NAFLD model in rats was established by giving a high-fat diet (HFD), ginsenoside-Rg1 was intragastrically administered 100 mg/kg/d for 8 weeks in NAFLD rat. Serum biochemical indices were measured Liver tissues were stained with hematoxylin and eosin (HE) and oil red O. Total RNA was extracted from liver and was used for high throughput sequencing to identify the changes of transcriptome. The relevant hub genes were verified by quantitative real-time PCR and western blot. KEY FINDINGS: Serum biochemical analysis indicated that ginsenoside-Rg1 improved liver function. Additionally, the staining of HE and oil red O indicated ginsenoside-Rg1 could remit pathology process of NAFLD. The transcriptome changes also support this result and reveals Atf3 and Acox2 were key genes. SIGNIFICANCE: Taken together, these results suggest that the efficiency of ginsenoside-Rg1 against NAFLD and confirmed that ginsenoside-Rg1 is a potential effective drug in treatment of NAFLD.
AIMS: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease and lacks for safe and effective drug to therapy completely. Ginsenoside-Rg1 is one of the main components of ginseng and has been proved to counteract a variety of diseases. However, there is currently a lack of sufficient evidence to support the efficacy of ginsenoside-Rg1 in the treatment of NAFLD. Our aim was to investigate whether Ginsenoside-Rg1 is a potential drug for NAFLD. MAIN METHODS: NAFLD model in rats was established by giving a high-fat diet (HFD), ginsenoside-Rg1 was intragastrically administered 100 mg/kg/d for 8 weeks in NAFLD rat. Serum biochemical indices were measured Liver tissues were stained with hematoxylin and eosin (HE) and oil red O. Total RNA was extracted from liver and was used for high throughput sequencing to identify the changes of transcriptome. The relevant hub genes were verified by quantitative real-time PCR and western blot. KEY FINDINGS: Serum biochemical analysis indicated that ginsenoside-Rg1 improved liver function. Additionally, the staining of HE and oil red O indicated ginsenoside-Rg1 could remit pathology process of NAFLD. The transcriptome changes also support this result and reveals Atf3 and Acox2 were key genes. SIGNIFICANCE: Taken together, these results suggest that the efficiency of ginsenoside-Rg1 against NAFLD and confirmed that ginsenoside-Rg1 is a potential effective drug in treatment of NAFLD.