Felipe Dominguez Machado1, Mirela Gehlen1, Vitória Schmidt Caron2, Gabriel Tassi Mousquer3, Graziele Lima Bello4, Camila Anton1, Rafaela Manzoni Bernardi1, Alana Ambos Freitas5, Gisela Unis6, Elis Regina Dalla Costa2, Maria Lucia Rosa Rossetti4, Denise Rossato Silva7,8,9. 1. Programa de Pós-Graduação em Ciências Pneumológicas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. 2. Centro de Desenvolvimento Científico e Tecnológico, Secretaria Estadual da Saúde do Rio Grande do Sul (CDCT/SES), Porto Alegre, RS, Brazil. 3. Programa de Pós-Graduação em Biociências da Universidade Federal de Ciências da Saúde de Porto Alegre (UFSCPA), Porto Alegre, RS, Brazil. 4. Programa de Pós-Graduação em Biologia Molecular e Celular Aplicada a Saúde (Biosaúde), Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil. 5. Faculdade de Medicina, Universidade Federal do Rio Grande do Sul (UFRGS), 2350 Ramiro Barcelos Street, Room 2050, Porto Alegre, RS, 90035-903, Brazil. 6. Hospital Sanatório Partenon, Porto Alegre, RS, Brazil. 7. Programa de Pós-Graduação em Ciências Pneumológicas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. denise.rossato@terra.com.br. 8. Faculdade de Medicina, Universidade Federal do Rio Grande do Sul (UFRGS), 2350 Ramiro Barcelos Street, Room 2050, Porto Alegre, RS, 90035-903, Brazil. denise.rossato@terra.com.br. 9. Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil. denise.rossato@terra.com.br.
Abstract
PURPOSE: The establishment of candidate genetic determinants associated with tuberculosis (TB) is a challenge, considering the divergent frequencies among populations. The objective of this study was to evaluate the association between MIF - 794 CATT 5-8 polymorphism and susceptibility to TB. METHODS: Case-control study. Patients > 18 years, with pulmonary TB were included. The control group consisted of blood donors and household contacts, not relatives, healthy and > 18 years. MIF - 794 CATT 5-8 were genotyped using sequencing of PCR and capillary electrophoresis. RESULTS: 126 patients and 119 controls were included. The genotype 5/5 was more frequent among cases (15.1%) than in controls (5.9%) (p = 0.019). Cases had more frequently the allele 5 (29.4%) as compared with controls (19.3%) (p = 0.010). Prevalence of 7/X + 8/X genotypes was not different between cases and controls (p = 0.821). There was no difference between patients with alleles 7 and 8 and those with alleles 5 and 6 (p = 0.608). CONCLUSIONS: The genotype 5/5 and the allele 5 of MIF - 794 CATT 5-8 were more frequent among TB patients than in controls.
PURPOSE: The establishment of candidate genetic determinants associated with tuberculosis (TB) is a challenge, considering the divergent frequencies among populations. The objective of this study was to evaluate the association between MIF - 794 CATT 5-8 polymorphism and susceptibility to TB. METHODS: Case-control study. Patients > 18 years, with pulmonary TB were included. The control group consisted of blood donors and household contacts, not relatives, healthy and > 18 years. MIF - 794 CATT 5-8 were genotyped using sequencing of PCR and capillary electrophoresis. RESULTS: 126 patients and 119 controls were included. The genotype 5/5 was more frequent among cases (15.1%) than in controls (5.9%) (p = 0.019). Cases had more frequently the allele 5 (29.4%) as compared with controls (19.3%) (p = 0.010). Prevalence of 7/X + 8/X genotypes was not different between cases and controls (p = 0.821). There was no difference between patients with alleles 7 and 8 and those with alleles 5 and 6 (p = 0.608). CONCLUSIONS: The genotype 5/5 and the allele 5 of MIF - 794 CATT 5-8 were more frequent among TB patients than in controls.
Authors: W Liu; W C Cao; C Y Zhang; L Tian; X M Wu; J D F Habbema; Q M Zhao; P H Zhang; Z T Xin; C Z Li; H Yang Journal: Int J Tuberc Lung Dis Date: 2004-04 Impact factor: 2.373