Catherine A Hernandez1, Andrea J Salazar1,2, Britt Koskella1. 1. Department of Integrative Biology, University of California, Berkeley, Berkeley, California, USA. 2. Chabot College, Hayward, California, USA.
Abstract
Background: One crucial first step in bacteriophage therapy is choosing a phage to apply, which involves screening for effectiveness in a meaningful way. Increasingly, research suggests that in vitro tests of phage-mediated bacterial lysis poorly translate to in planta effectiveness. Materials and Methods: We tested a seedling-based method for rapidly screening phage effectiveness in vivo. In three trials, phages were prophylactically applied to tomato seedlings in sterile conical tubes before flooding with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. We recorded seedling disease progression and quantified endpoint bacteria and phage densities. Results: Phages replicated in all trials, but reduction of disease symptoms and endpoint P. syringae density varied across trials with different application densities. Conclusions: This resource-efficient method rapidly identified an effective phage and application density to mitigate disease on seedlings. We propose that this method could be used to screen candidate phages before testing in agricultural conditions. Copyright 2020, Mary Ann Liebert, Inc., publishers.
Background: One crucial first step in bacteriophage therapy is choosing a phage to apply, which involves screening for effectiveness in a meaningful way. Increasingly, research suggests that in vitro tests of phage-mediated bacterial lysis poorly translate to in planta effectiveness. Materials and Methods: We tested a seedling-based method for rapidly screening phage effectiveness in vivo. In three trials, phages were prophylactically applied to tomato seedlings in sterile conical tubes before flooding with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. We recorded seedling disease progression and quantified endpoint bacteria and phage densities. Results: Phages replicated in all trials, but reduction of disease symptoms and endpoint P. syringae density varied across trials with different application densities. Conclusions: This resource-efficient method rapidly identified an effective phage and application density to mitigate disease on seedlings. We propose that this method could be used to screen candidate phages before testing in agricultural conditions. Copyright 2020, Mary Ann Liebert, Inc., publishers.
Authors: Cindy E Morris; David C Sands; Boris A Vinatzer; Catherine Glaux; Caroline Guilbaud; Alain Buffière; Shuangchun Yan; Hélène Dominguez; Brian M Thompson Journal: ISME J Date: 2008-01-10 Impact factor: 10.302