Yarrowia is a yeast genus that has been used as a model oleaginous taxon for a wide array of studies. However, information regarding metabolite changes within Yarrowia spp. under different environmental conditions is still limited. Among various factors affecting Yarrowia metabolism, nitrogen-limiting conditions have a profound effect on the metabolic state of yeast. In this study, a time-course LC-MS/MS-based metabolome analysis of Y. lipolytica was performed to determine the optimal cultivation time and carbon-to-nitrogen ratio for studying the effects of nitrogen-limiting conditions on Yarrowia; we found that cultivation time of 36 h and carbon-to-nitrogen ratio of 4:1 and 5:0 was suitable for studying the effects of nitrogen-limiting conditions on Yarrowia and these conditions were applied to six strains of Yarrowia. These six strains of Yarrowia showed similar responses to nitrogen-limiting conditions; however, each strain had a unique metabolomic profile. Purine and pyrimidine metabolism were the most highly affected biological pathways in nitrogen-limiting conditions, indicating that these conditions affect energy availability within cells. This stress leads to a shift in cells to the utilization of a less ATP-dependent biological pathway. This information will be beneficial for the development of Yarrowia strains for further scientific and industrial applications.
Yarrowia is a n class="Species">yeast genus that has been used as a model oleaginous taxon for a wide array of studies. However, information regarding metabolite changes within Yarrowia spp. under different environmental conditions is still limited. Among various factors affecting Yarrowia metabolism, nitrogen-limiting conditions have a profound effect on the metabolic state of yeast. In this study, a time-course LC-MS/MS-based metabolome analysis of Y. lipolytica was performed to determine the optimal cultivation time and carbon-to-nitrogen ratio for studying the effects of nitrogen-limiting conditions on Yarrowia; we found that cultivation time of 36 h and carbon-to-nitrogen ratio of 4:1 and 5:0 was suitable for studying the effects of nitrogen-limiting conditions on Yarrowia and these conditions were applied to six strains of Yarrowia. These six strains of Yarrowia showed similar responses to nitrogen-limiting conditions; however, each strain had a unique metabolomic profile. Purine and pyrimidine metabolism were the most highly affected biological pathways in nitrogen-limiting conditions, indicating that these conditions affect energy availability within cells. This stress leads to a shift in cells to the utilization of a less ATP-dependent biological pathway. This information will be beneficial for the development of Yarrowia strains for further scientific and industrial applications.
Authors: P W Crous; M J Wingfield; T I Burgess; A J Carnegie; G E St J Hardy; D Smith; B A Summerell; J F Cano-Lira; J Guarro; J Houbraken; L Lombard; M P Martín; M Sandoval-Denis; A V Alexandrova; C W Barnes; I G Baseia; J D P Bezerra; V Guarnaccia; T W May; M Hernández-Restrepo; A M Stchigel; A N Miller; M E Ordoñez; V P Abreu; T Accioly; C Agnello; A Agustin Colmán; C C Albuquerque; D S Alfredo; P Alvarado; G R Araújo-Magalhães; S Arauzo; T Atkinson; A Barili; R W Barreto; J L Bezerra; T S Cabral; F Camello Rodríguez; R H S F Cruz; P P Daniëls; B D B da Silva; D A C de Almeida; A A de Carvalho Júnior; C A Decock; L Delgat; S Denman; R A Dimitrov; J Edwards; A G Fedosova; R J Ferreira; A L Firmino; J A Flores; D García; J Gené; A Giraldo; J S Góis; A A M Gomes; C M Gonçalves; D E Gouliamova; M Groenewald; B V Guéorguiev; M Guevara-Suarez; L F P Gusmão; K Hosaka; V Hubka; S M Huhndorf; M Jadan; Ž Jurjević; B Kraak; V Kučera; T K A Kumar; I Kušan; S R Lacerda; S Lamlertthon; W S Lisboa; M Loizides; J J Luangsa-Ard; P Lysková; W P Mac Cormack; D M Macedo; A R Machado; E F Malysheva; P Marinho; N Matočec; M Meijer; A Mešić; S Mongkolsamrit; K A Moreira; O V Morozova; K U Nair; N Nakamura; W Noisripoom; I Olariaga; R J V Oliveira; L M Paiva; P Pawar; O L Pereira; S W Peterson; M Prieto; E Rodríguez-Andrade; C Rojo De Blas; M Roy; E S Santos; R Sharma; G A Silva; C M Souza-Motta; Y Takeuchi-Kaneko; C Tanaka; A Thakur; M Th Smith; Z Tkalčec; N Valenzuela-Lopez; P van der Kleij; A Verbeken; M G Viana; X W Wang; J Z Groenewald Journal: Persoonia Date: 2017-12-20 Impact factor: 11.051
Authors: Ann Kristin Knutsen; V Robert; G A Poot; Wendy Epping; Marian Figge; A Holst-Jensen; Ida Skaar; Maudy Th Smith Journal: Int J Syst Evol Microbiol Date: 2007-10 Impact factor: 2.747