Zuzana Vrablicova1, Kristina Tomova2, Lubomira Tothova3, Janka Babickova3,4, Barbora Gromova3, Barbora Konecna3, Robert Liptak5, Tibor Hlavaty1, Roman Gardlik3. 1. 5th Department of Internal Medicine, Sub-department of Gastroenterology and Hepatology, Faculty of Medicine, Comenius University, University Hospital Bratislava, Bratislava, Slovakia. 2. 2nd Department of Gynecology and Obstetrics, Hospital of F. D. Roosevelt, Slovak Medical University, Banska Bystrica, Slovakia. 3. Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia. 4. Department of Clinical Medicine, University of Bergen, Bergen, Norway. 5. Faculty of Medicine, Institute of Physiology, Comenius University, Bratislava, Slovakia.
Abstract
Background: The role of cell-free DNA (cfDNA) in the pathogenesis of inflammatory bowel disease (IBD) has been recently suggested. The aim of this study was to analyze circulating cfDNA and deoxyribonuclease (DNase) activity in IBD patients in clinical remission. Materials and Methods: Plasma and serum were obtained from 72 patients with Crohn's disease and 28 patients with ulcerative colitis. Total cfDNA, nuclear DNA (ncDNA), mitochondrial DNA (mtDNA) and DNase activity were measured. Results: IBD patients showed higher levels of both ncDNA and mtDNA compared to healthy controls. Concentration of ncDNA was higher in males compared to females, including patients and healthy controls. However, unlike males higher amount of ncDNA was found in female IBD patients compared to healthy controls. DNase activity was significantly lower in male IBD patients compared with healthy controls. In addition, there was a negative correlation between DNase activity and ncDNA levels in male IBD patients. Conclusions: Herein we present increased amount of circulating ncDNA and mtDNA in IBD patients in clinical remission. Thus, unlike total cfDNA, circulating ncDNA and mtDNA might not represent the optimal biomarkers of disease activity. This is also the first report on sex difference in circulating ncDNA levels, possibly associated with lower DNase activity in males.
Background: The role of cell-free DNA (cfDNA) in the pathogenesis of inflammatory bowel disease (IBD) has been recently suggested. The aim of this study was to analyze circulating cfDNA and deoxyribonuclease (DNase) activity in IBDpatients in clinical remission. Materials and Methods: Plasma and serum were obtained from 72 patients with Crohn's disease and 28 patients with ulcerative colitis. Total cfDNA, nuclear DNA (ncDNA), mitochondrial DNA (mtDNA) and DNase activity were measured. Results:IBDpatients showed higher levels of both ncDNA and mtDNA compared to healthy controls. Concentration of ncDNA was higher in males compared to females, including patients and healthy controls. However, unlike males higher amount of ncDNA was found in female IBDpatients compared to healthy controls. DNase activity was significantly lower in male IBDpatients compared with healthy controls. In addition, there was a negative correlation between DNase activity and ncDNA levels in male IBDpatients. Conclusions: Herein we present increased amount of circulating ncDNA and mtDNA in IBDpatients in clinical remission. Thus, unlike total cfDNA, circulating ncDNA and mtDNA might not represent the optimal biomarkers of disease activity. This is also the first report on sex difference in circulating ncDNA levels, possibly associated with lower DNase activity in males.
Authors: Hayley J MacKinnon; Teodora R Kolarova; Ronit Katz; Jaclynne M Hedge; Elena Vinopal; Christina M Lockwood; Raj Shree; Shani Delaney Journal: Am J Obstet Gynecol MFM Date: 2021-08-18
Authors: Huiwen Che; Tatjana Jatsenko; Lore Lannoo; Kate Stanley; Luc Dehaspe; Leen Vancoillie; Nathalie Brison; Ilse Parijs; Kris Van Den Bogaert; Koenraad Devriendt; Sabien Severi; Ellen De Langhe; Severine Vermeire; Bram Verstockt; Kristel Van Calsteren; Joris Robert Vermeesch Journal: NPJ Genom Med Date: 2022-09-14 Impact factor: 6.083