Rediet Tesfaye1, Abel Degu1, Besufekad Abebe2, Hiwot Ayalew2. 1. Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia. 2. Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Abstract
INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice.
INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice.
Authors: Jennifer L St Sauver; David O Warner; Barbara P Yawn; Debra J Jacobson; Michaela E McGree; Joshua J Pankratz; L Joseph Melton; Véronique L Roger; Jon O Ebbert; Walter A Rocca Journal: Mayo Clin Proc Date: 2013-01 Impact factor: 7.616