Carmen Delianu1,2, Mihaela Moscalu3, Loredana Liliana Hurjui2,4, Claudia Cristina Tărniceriu5,6, Oana-Viola Bădulescu4,6, Ludmila Lozneanu7,8, Ion Hurjui9, Ancuta Goriuc1, Zinovia Surlari10, Liliana Foia1,11. 1. Department of Biochemistry, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. 2. Central Clinical Laboratory-Hematology Department, "Sf. Spiridon" County Clinical Emergency Hospital, 700111 Iasi, Romania. 3. Department of Preventive Medicine and Interdisciplinarity, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. 4. Department of Morpho-Functional Sciences II, Discipline of Physiology, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. 5. Department of Morpho-Functional Sciences I, Discipline of Anatomy, "Grigore T. Popa" University of Medicine and Pharmacy, Universității str. 16, 700115 Iasi, Romania. 6. Hematology Clinic, "Sf. Spiridon" County Clinical Emergency Hospital, 700111 Iasi, Romania. 7. Department of Morpho-Functional Sciences I, Discipline of Histology, "Grigore T. Popa" University of Medicine and Pharmacy, Universității str. 16, 700115 Iasi, Romania. 8. Department of Pathology, "Sf. Spiridon" Emergency County Hospital, 700111 Iasi, Romania. 9. Department of Morpho-Functional Sciences II, Discipline of Biophysics, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. 10. Department of Odontology and Parodontology, "Grigore T. Popa" University of Medicine and Pharmacy, Universității str. 16, 700115 Iasi, Romania. 11. Central Clinical Laboratory-Biochemistry Department, "Sf. Spiridon" County Clinical Emergency Hospital, 700111 Iasi, Romania.
Abstract
Prolonged tourniquet stasis induced by venepuncture can lead to the release of the plasma of cell lysis products, as well as tissue factor (TF), impairing the quality of coagulation test results. The accidental presence of TF in vitro can trigger the coagulation mechanism, generating a false decrease in prothrombin time (PT). Background and Objectives: Identification of short PT tests below the normal reference value that could suggest a situation of hypercoagulability. The study aimed to compare the results of the shortened PT tests at their first determination with the eventual correction following duplication of the analysis from the same sample. Materials and methods: Identification of the shortened PT tests has been carried out for a period of 4 months, upon 544 coagulation samples referred to the Hematology department of Sf. Spiridon County Clinical Emergency Hospital from Iasi, Romania. Results: Out of the 544 samples of which the results indicated a state of hypercoagulability, by repeating the determination from the same sample, for 200 (36.76%) PT tests (p = 0.001) the value was corrected, falling within the normal reference range. For 344 (63.24%) tests, the results suggested a situation of hypercoagulability. Conclusions: In order to guarantee the highest quality of the laboratory services, a proper interpretation and report of the patients' results must be congruent and harmoniously associated to the actual clinical condition of the patient. Duplication of the PT determination from the same sample would exclude situations of false hypercoagulability and would provide significant improvement for the patient's safety.
Prolonged tourniquet stasis induced by venepuncture can lead to the release of the plasma of cell lysis products, as well as tissue factor (TF), impairing the quality of coagulation test results. The accidental presence of TF in vitro can trigger the coagulation mechanism, generating a false decrease in prothrombin time (PT). Background and Objectives: Identification of short PT tests below the normal reference value that could suggest a situation of hypercoagulability. The study aimed to compare the results of the shortened PT tests at their first determination with the eventual correction following duplication of the analysis from the same sample. Materials and methods: Identification of the shortened PT tests has been carried out for a period of 4 months, upon 544 coagulation samples referred to the Hematology department of Sf. Spiridon County Clinical Emergency Hospital from Iasi, Romania. Results: Out of the 544 samples of which the results indicated a state of hypercoagulability, by repeating the determination from the same sample, for 200 (36.76%) PT tests (p = 0.001) the value was corrected, falling within the normal reference range. For 344 (63.24%) tests, the results suggested a situation of hypercoagulability. Conclusions: In order to guarantee the highest quality of the laboratory services, a proper interpretation and report of the patients' results must be congruent and harmoniously associated to the actual clinical condition of the patient. Duplication of the PT determination from the same sample would exclude situations of false hypercoagulability and would provide significant improvement for the patient's safety.
Authors: Giuseppe Lippi; Jeffrey J Chance; Stephen Church; Paola Dazzi; Rossana Fontana; Davide Giavarina; Kjell Grankvist; Wim Huisman; Timo Kouri; Vladimir Palicka; Mario Plebani; Vincenzo Puro; Gian Luca Salvagno; Sverre Sandberg; Ken Sikaris; Ian Watson; Ana K Stankovic; Ana-Maria Simundic Journal: Clin Chem Lab Med Date: 2011-04-25 Impact factor: 3.694
Authors: Nabil G Guirguis; Chad Eicher; Lynette Hock; James Lynch; Vicki D Graham; P R Rajagopalan; Amal Guirguis; John Lazarchick Journal: Ann Clin Lab Sci Date: 2003 Impact factor: 1.256