Literature DB >> 33377046

An Improved Protocol for the Production of Lentiviral Vectors.

Logan Y Brown1, Wendy Dong1, Boris Kantor1,2.   

Abstract

Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
© 2020 The Authors.

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Year:  2020        PMID： 33377046      PMCID： PMC7757317          DOI： 10.1016/j.xpro.2020.100152

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  5 in total

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Journal:  Annu Rev Biomed Eng       Date:  2008       Impact factor: 9.590

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Journal:  J Vis Exp       Date:  2017-12-12       Impact factor: 1.355

3.  Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models.

Authors:  Lidia Tagliafierro; Ekaterina Ilich; Malik Moncalvo; Jeffrey Gu; Ahila Sriskanda; Carole Grenier; Susan K Murphy; Ornit Chiba-Falek; Boris Kantor
Journal:  J Vis Exp       Date:  2019-03-29       Impact factor: 1.355

4.  A third-generation lentivirus vector with a conditional packaging system.

Authors:  T Dull; R Zufferey; M Kelly; R J Mandel; M Nguyen; D Trono; L Naldini
Journal:  J Virol       Date:  1998-11       Impact factor: 5.103

5.  Integrase-Deficient Lentiviral Vector as an All-in-One Platform for Highly Efficient CRISPR/Cas9-Mediated Gene Editing.

Authors:  Pavel I Ortinski; Bernadette O'Donovan; Xiaoyu Dong; Boris Kantor
Journal:  Mol Ther Methods Clin Dev       Date:  2017-04-19       Impact factor: 6.698
  5 in total
  6 in total

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  6 in total

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