Literature DB >> 33377043

Identification and Functional Analysis of Human CD56neg NK Cells by Flow Cytometry.

Ane Orrantia1, Iñigo Terrén1, Joana Vitallé1, Gabirel Astarloa-Pando1, Olatz Zenarruzabeitia1, Francisco Borrego1,2.   

Abstract

Although scarce in the peripheral blood of healthy people, CD56neg NK cells are known to be expanded in some pathological conditions. However, studies on CD56neg NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for the identification and functional analysis of CD56neg NK cells, providing an improved gating strategy for the selection of this intriguing population. For complete details on the use and execution of this protocol, please refer to Orrantia et al. (2020).
© 2020 The Author(s).

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Year:  2020        PMID： 33377043      PMCID： PMC7757323          DOI： 10.1016/j.xpro.2020.100149

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  9 in total

1.  Sequential deregulation of NK cell subset distribution and function starting in acute HIV-1 infection.

Authors:  Galit Alter; Nickolas Teigen; Benjamin T Davis; Marylyn M Addo; Todd J Suscovich; Michael T Waring; Hendrik Streeck; Mary N Johnston; Kyle D Staller; M Tauheed Zaman; Xu G Yu; Mathias Lichterfeld; Nesli Basgoz; Eric S Rosenberg; Marcus Altfeld
Journal:  Blood       Date:  2005-07-07       Impact factor: 22.113

2.  NKp80 defines and stimulates a reactive subset of CD8 T cells.

Authors:  Sabrina Kuttruff; Sven Koch; Alexandra Kelp; Graham Pawelec; Hans-Georg Rammensee; Alexander Steinle
Journal:  Blood       Date:  2008-10-15       Impact factor: 22.113

Review 3.  Innate Lymphoid Cells: 10 Years On.

Authors:  Eric Vivier; David Artis; Marco Colonna; Andreas Diefenbach; James P Di Santo; Gérard Eberl; Shigeo Koyasu; Richard M Locksley; Andrew N J McKenzie; Reina E Mebius; Fiona Powrie; Hergen Spits
Journal:  Cell       Date:  2018-08-23       Impact factor: 41.582

4.  Effect of age and CMV on NK cell subpopulations.

Authors:  Carmen Campos; Alejandra Pera; Beatriz Sanchez-Correa; Corona Alonso; Isabel Lopez-Fernandez; Sara Morgado; Raquel Tarazona; Rafael Solana
Journal:  Exp Gerontol       Date:  2014-01-17       Impact factor: 4.032

5.  Natural killer cell immunodeficiency in HIV disease is manifest by profoundly decreased numbers of CD16+CD56+ cells and expansion of a population of CD16dimCD56- cells with low lytic activity.

Authors:  P F Hu; L E Hultin; P Hultin; M A Hausner; K Hirji; A Jewett; B Bonavida; R Detels; J V Giorgi
Journal:  J Acquir Immune Defic Syndr Hum Retrovirol       Date:  1995-11-01

6.  Genetically coupled receptor-ligand pair NKp80-AICL enables autonomous control of human NK cell responses.

Authors:  Sascha N Klimosch; Yvonne Bartel; Stefan Wiemann; Alexander Steinle
Journal:  Blood       Date:  2013-08-08       Impact factor: 22.113

7.  A NKp80-Based Identification Strategy Reveals that CD56neg NK Cells Are Not Completely Dysfunctional in Health and Disease.

Authors:  Ane Orrantia; Iñigo Terrén; Alicia Izquierdo-Lafuente; Juncal A Alonso-Cabrera; Victor Sandá; Joana Vitallé; Santiago Moreno; María Tasias; Alasne Uranga; Carmen González; Juan J Mateos; Juan C García-Ruiz; Olatz Zenarruzabeitia; Francisco Borrego
Journal:  iScience       Date:  2020-06-20

8.  CD300a inhibits CD16-mediated NK cell effector functions in HIV-1-infected patients.

Authors:  Joana Vitallé; Iñigo Terrén; Ane Orrantia; Raquel Pérez-Garay; Francesc Vidal; José A Iribarren; Carmen Rodríguez; Ana M López Lirola; Enrique Bernal; Olatz Zenarruzabeitia; Francisco Borrego
Journal:  Cell Mol Immunol       Date:  2019-08-29       Impact factor: 11.530

9.  CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age.

Authors:  Bojana Müller-Durovic; Jasmin Grählert; Oliver P Devine; Arne N Akbar; Christoph Hess
Journal:  Aging (Albany NY)       Date:  2019-01-27       Impact factor: 5.682
  9 in total
  5 in total

1.  Role of NK Cells in Tumor Progression.

Authors:  Iñigo Terrén; Francisco Borrego
Journal:  Exp Suppl       Date:  2022

2.  T Cell Activation, Highly Armed Cytotoxic Cells and a Shift in Monocytes CD300 Receptors Expression Is Characteristic of Patients With Severe COVID-19.

Authors:  Olatz Zenarruzabeitia; Gabirel Astarloa-Pando; Iñigo Terrén; Ane Orrantia; Raquel Pérez-Garay; Iratxe Seijas-Betolaza; Javier Nieto-Arana; Natale Imaz-Ayo; Silvia Pérez-Fernández; Eunate Arana-Arri; Francisco Borrego
Journal:  Front Immunol       Date:  2021-03-11       Impact factor: 7.561

3.  NK Cell Reconstitution After Autologous Hematopoietic Stem Cell Transplantation: Association Between NK Cell Maturation Stage and Outcome in Multiple Myeloma.

Authors:  Ane Orrantia; Iñigo Terrén; Gabirel Astarloa-Pando; Carmen González; Alasne Uranga; Juan J Mateos-Mazón; Juan C García-Ruiz; Marta Riñón; Mercedes Rey; Silvia Pérez-Fernandez; Olatz Zenarruzabeitia; Francisco Borrego
Journal:  Front Immunol       Date:  2021-10-05       Impact factor: 7.561

Review 4.  Human NK Cells in Autologous Hematopoietic Stem Cell Transplantation for Cancer Treatment.

Authors:  Ane Orrantia; Iñigo Terrén; Gabirel Astarloa-Pando; Olatz Zenarruzabeitia; Francisco Borrego
Journal:  Cancers (Basel)       Date:  2021-03-30       Impact factor: 6.639

5.  Immune Dysfunctions of CD56neg NK Cells Are Associated With HIV-1 Disease Progression.

Authors:  Wen-Jing Cao; Xiao-Chang Zhang; Lin-Yu Wan; Qing-Yu Li; Xiu-Ying Mu; An-Liang Guo; Ming-Ju Zhou; Li-Li Shen; Chao Zhang; Xing Fan; Yan-Mei Jiao; Ruo-Nan Xu; Chun-Bao Zhou; Jin-Hong Yuan; Sheng-Qi Wang; Fu-Sheng Wang; Jin-Wen Song
Journal:  Front Immunol       Date:  2022-01-07       Impact factor: 7.561
  5 in total

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