| Literature DB >> 33377041 |
Renato Socodato1, Pedro Melo1, José P Ferraz-Nogueira1, Camila C Portugal1, João B Relvas1,2.
Abstract
This protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).Entities:
Mesh：
Year: 2020 PMID： 33377041 PMCID： PMC7757332 DOI： 10.1016/j.xpro.2020.100147
Source DB: PubMed Journal: STAR Protoc ISSN： 2666-1667