PURPOSE: In order to study novel therapeutic approaches taking advantage of natural compounds showing anticancer and anti-proliferative effects, we focused our interest on S-adenosyl-l-methionine, a naturally occurring sulfur-containing nucleoside synthesized from adenosine triphosphate and methionine by methionine adenosyltransferase, and its potential in overcoming drug resistance in colon cancer cells devoid of p53. RESULTS: In the present study, we demonstrated that S-adenosyl-l-methionine overcomes uL3-mediated drug resistance in p53 deleted colon cancer cells. In particular, we demonstrated that S-adenosyl-l-methionine causes cell cycle arrest at the S phase; inhibits autophagy; augments reactive oxygen species; and induces apoptosis in these cancer cells. CONCLUSIONS: Results reported in this paper led us to propose S-adenosyl-l-methionine as a potential promising agent for cancer therapy by examining p53 and uL3 profiles in tumors to yield a better clinical outcomes.
PURPOSE: In order to study novel therapeutic approaches taking advantage of natural compounds showing anticancer and anti-proliferative effects, we focused our interest on S-adenosyl-l-methionine, a naturally occurring sulfur-containing nucleoside synthesized from adenosine triphosphate and methionine by methionine adenosyltransferase, and its potential in overcoming drug resistance in colon cancer cells devoid of p53. RESULTS: In the present study, we demonstrated that S-adenosyl-l-methionine overcomes uL3-mediated drug resistance in p53 deleted colon cancer cells. In particular, we demonstrated that S-adenosyl-l-methionine causes cell cycle arrest at the S phase; inhibits autophagy; augments reactive oxygen species; and induces apoptosis in these cancer cells. CONCLUSIONS: Results reported in this paper led us to propose S-adenosyl-l-methionine as a potential promising agent for cancer therapy by examining p53 and uL3 profiles in tumors to yield a better clinical outcomes.
Entities:
Keywords:
AdoMet; apoptosis; autophagy; colon cancer; drug resistance; uL3
Authors: David Fernández-Ramos; Fernando Lopitz-Otsoa; Laura Delacruz-Villar; Jon Bilbao; Martina Pagano; Laura Mosca; Maider Bizkarguenaga; Marina Serrano-Macia; Mikel Azkargorta; Marta Iruarrizaga-Lejarreta; Jesús Sot; Darya Tsvirkun; Sebastiaan Martijn van Liempd; Felix M Goni; Cristina Alonso; María Luz Martínez-Chantar; Felix Elortza; Liat Hayardeny; Shelly C Lu; José M Mato Journal: World J Gastroenterol Date: 2020-09-14 Impact factor: 5.742
Authors: Luigi Borzacchiello; Roberta Veglia Tranchese; Roberta Grillo; Roberta Arpino; Laura Mosca; Giovanna Cacciapuoti; Marina Porcelli Journal: Int J Mol Sci Date: 2022-07-12 Impact factor: 6.208