Hanri Zeng1, Lina Yi2, Xiaoli Chen3, Huiqiong Zhou1, Huanying Zheng1, Jing Lu2, Fen Yang1, Caixia Li1, Ling Fang1, Xin Zhang1, Xu Jing1, Jie Wu1, Hui Li4. 1. Center for Disease Control and Prevention of Guangdong Province, Guangzhou, Guangdong, China. 2. Guangdong Provincial Institute of Public Health, Guangdong Provincial Centre for Disease Control and Prevention, China. 3. Laboratory Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, China. 4. Center for Disease Control and Prevention of Guangdong Province, Guangzhou, Guangdong, China. Electronic address: gzli_hui@tom.com.
Abstract
BACKGROUND: EV-A71 is a common causative agent of hand foot and mouth disease. In mainland China, EV-A71 subgenotype C4 has been the sole circulating genotype since 2008, and was used in the production of multiple licensed vaccines. Here, we report the first detection EV-A71 C1 strains in China. METHODS: Full genomic sequence were obtained. The origin of the EV-A71 C1 strains were tracked down by Bayesian inferences. Recombination was analyzed using Simplot program. And the antigenicity were tested using the microneutralization test. RESULTS: The C1-GD2019 shared high identity with the C1-like lineage recently identified in Europe and was introduced into Guangdong in 2018-2019. Close genetic relatedness between the C1-GD2019 and Europe C1-like strains were observed except for the 3D-3'UTR region. The late showed high similarity with CVA genomes. Antigenic variance was found. The C1-GD2019 could not be effectively neutralized by EV-A71 C4a neutralizing antibody positive samples. CONCLUSION: This is the first report of EV-A71 subgenotype C1 isolated in China. It is a recombinant strain originating from C1-like strains recently identified in Europe and CVA strains. The different antigenicity between the C1 strains and C4a vaccine strains highlighted the importance on closely monitoring the EV-A71 C1 strains in China.
BACKGROUND:EV-A71 is a common causative agent of hand foot and mouth disease. In mainland China, EV-A71 subgenotype C4 has been the sole circulating genotype since 2008, and was used in the production of multiple licensed vaccines. Here, we report the first detection EV-A71 C1 strains in China. METHODS: Full genomic sequence were obtained. The origin of the EV-A71 C1 strains were tracked down by Bayesian inferences. Recombination was analyzed using Simplot program. And the antigenicity were tested using the microneutralization test. RESULTS: The C1-GD2019 shared high identity with the C1-like lineage recently identified in Europe and was introduced into Guangdong in 2018-2019. Close genetic relatedness between the C1-GD2019 and Europe C1-like strains were observed except for the 3D-3'UTR region. The late showed high similarity with CVA genomes. Antigenic variance was found. The C1-GD2019 could not be effectively neutralized by EV-A71 C4a neutralizing antibody positive samples. CONCLUSION: This is the first report of EV-A71 subgenotype C1 isolated in China. It is a recombinant strain originating from C1-like strains recently identified in Europe and CVA strains. The different antigenicity between the C1 strains and C4a vaccine strains highlighted the importance on closely monitoring the EV-A71 C1 strains in China.
Authors: V Spagnuolo; M Guffanti; L Galli; A Poli; P Rovere Querini; M Ripa; M Clementi; P Scarpellini; A Lazzarin; M Tresoldi; L Dagna; A Zangrillo; F Ciceri; A Castagna Journal: Sci Rep Date: 2020-12-04 Impact factor: 4.379
Authors: Lei Liu; Qiu Xi Zeng; Hang Qu; Jun Jian Li; Yan Wei Yang; Yan Wen Gong; Zhong Zhi He; Yi He Zhang; Wei Zhang; Bin Liu; Li Chun Che Journal: Clin Exp Med Date: 2021-10-22 Impact factor: 5.057