Literature DB >> 33370878

Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions.

Cécile Méjécase1, Philippa Harding2, Hajrah Sarkar1, Jonathan Eintracht2, Dulce Lima Cunha2, Lyes Toualbi1, Mariya Moosajee3.   

Abstract

Two human induced pluripotent stem cell (hiPSC) lines (UCLi016-A and UCLi017-A) were generated from fibroblast cells of 23- and 34-year-old healthy male donors with no known ocular conditions. Fibroblast cells were derived from skin biopsies and reprogrammed using integration free episomal reprogramming. The established iPSC lines were found to express pluripotency markers, exhibit differentiation potential in vitro and display a normal karyotype. These cell lines will act as a control lines for researchers studying ocular diseases.
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

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Year:  2020        PMID： 33370878      PMCID： PMC7768179          DOI： 10.1016/j.scr.2020.102113

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  3 in total

1.  Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

Authors:  Jian Ye; George Coulouris; Irena Zaretskaya; Ioana Cutcutache; Steve Rozen; Thomas L Madden
Journal:  BMC Bioinformatics       Date:  2012-06-18       Impact factor: 3.169

2.  DNA copy number analysis of fresh and formalin-fixed specimens by shallow whole-genome sequencing with identification and exclusion of problematic regions in the genome assembly.

Authors:  Ilari Scheinin; Daoud Sie; Henrik Bengtsson; Mark A van de Wiel; Adam B Olshen; Hinke F van Thuijl; Hendrik F van Essen; Paul P Eijk; François Rustenburg; Gerrit A Meijer; Jaap C Reijneveld; Pieter Wesseling; Daniel Pinkel; Donna G Albertson; Bauke Ylstra
Journal:  Genome Res       Date:  2014-09-18       Impact factor: 9.043

3.  Identification and Correction of Mechanisms Underlying Inherited Blindness in Human iPSC-Derived Optic Cups.

Authors:  David A Parfitt; Amelia Lane; Conor M Ramsden; Amanda-Jayne F Carr; Peter M Munro; Katarina Jovanovic; Nele Schwarz; Naheed Kanuga; Manickam N Muthiah; Sarah Hull; Jean-Marc Gallo; Lyndon da Cruz; Anthony T Moore; Alison J Hardcastle; Peter J Coffey; Michael E Cheetham
Journal:  Cell Stem Cell       Date:  2016-04-14       Impact factor: 24.633
  3 in total
  2 in total

1.  hiPSC-Derived Epidermal Keratinocytes from Ichthyosis Patients Show Altered Expression of Cornification Markers.

Authors:  Dulce Lima Cunha; Amanda Oram; Robert Gruber; Roswitha Plank; Arno Lingenhel; Manoj K Gupta; Janine Altmüller; Peter Nürnberg; Matthias Schmuth; Johannes Zschocke; Tomo Šarić; Katja M Eckl; Hans C Hennies
Journal:  Int J Mol Sci       Date:  2021-02-11       Impact factor: 5.923

2.  Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells.

Authors:  Jonathan Eintracht; Philippa Harding; Dulce Lima Cunha; Mariya Moosajee
Journal:  F1000Res       Date:  2022-03-17
  2 in total

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