| Literature DB >> 33368000 |
Mohammed Kaplan1, William J Nicolas1,2, Wei Zhao1,2, Stephen D Carter1, Lauren Ann Metskas1,2, Georges Chreifi1, Debnath Ghosal1,3, Grant J Jensen4,5,6.
Abstract
Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact macromolecular complexes while they are in their cellular milieu. Over the years, cryo-ET has had a huge impact on our understanding of how large biomolecular complexes look like, how they assemble, disassemble, function, and evolve(d). Recent hardware and software developments and combining cryo-ET with other techniques, e.g., focused ion beam milling (FIB-milling) and cryo-light microscopy, has extended the realm of cryo-ET to include transient molecular complexes embedded deep in thick samples (like eukaryotic cells) and enhanced the resolution of structures obtained by cryo-ET. In this chapter, we will present an outline of how to perform cryo-ET studies on a wide variety of biological samples including prokaryotic and eukaryotic cells and biological plant tissues. This outline will include sample preparation, data collection, and data processing as well as hybrid approaches like FIB-milling, cryosectioning, and cryo-correlated light and electron microscopy (cryo-CLEM).Keywords: Cryo-ET; Cryosectioning; FIB-milling; High-resolution tomography; In situ structural biology; Subtomogram averaging
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Year: 2021 PMID： 33368000 DOI： 10.1007/978-1-0716-0966-8_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745