Travis M Griffiths1, Lauren Page1, Andrew S Weyrich2, Matthew T Rondina3, Robert A Campbell4. 1. University of Utah Molecular Medicine Program, Salt Lake City, UT 84112, United States of America. 2. University of Utah Molecular Medicine Program, Salt Lake City, UT 84112, United States of America; Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, United States of America. 3. University of Utah Molecular Medicine Program, Salt Lake City, UT 84112, United States of America; Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, United States of America; George E. Wahlen VAMC Department of Internal Medicine and GRECC, Salt Lake City, UT 84148, United States of America; Department of Pathology, University of Utah, Salt Lake City, UT 84132, United States of America. 4. University of Utah Molecular Medicine Program, Salt Lake City, UT 84112, United States of America; Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, United States of America. Electronic address: rcampbell@u2m2.utah.edu.
Abstract
BACKGROUND: The ability to measure changes in platelet reactivity is important to identify novel aspects of platelet biology and develop targeted therapeutics to prevent bleeding or thrombosis. Current platelet function testing allows for single agonist analysis at a time. The ability to phenotype platelets in a single assay with multiple agonists and adhesion substrates could yield more insights into altered pathways than are feasible with current approaches. We hypothesized platelet electrical resistance (PER) could be used for more comprehensive phenotyping of platelets. METHODS: Platelets were isolated from male and female healthy donors (age 39.6 ± 6.9) and septic patients (age 44.0 ± 13.5). PER 96-well plates were coated with various substrates, including fibrinogen and collagen. Platelets were added to the coated plates in the presence or absence of thrombin or convulxin. Platelet activation and spreading was monitored by measuring changes in electrical impedance. RESULTS: Platelets adhesion to fibrinogen and collagen increased impedance. In addition, impedance increased in response to thrombin or convulxin. No changes in impedance were observed in the absence of platelets or when wells were uncoated, indicating changes in impedance were directly due to platelet adhesion and activation. Inhibiting integrin αIIbβ3 decreased impedance when fibrinogen was used as a substrate, consistent with platelet-dependent effects. Platelets from septic patients caused increased impedance compared to healthy donors, demonstrating this assay can be used to assess platelet hyperreactivity. CONCLUSION: PER can be applied as a high throughput tool to measure platelet reactivity in health and disease, where platelet activation is increased.
BACKGROUND: The ability to measure changes in platelet reactivity is important to identify novel aspects of platelet biology and develop targeted therapeutics to prevent bleeding or thrombosis. Current platelet function testing allows for single agonist analysis at a time. The ability to phenotype platelets in a single assay with multiple agonists and adhesion substrates could yield more insights into altered pathways than are feasible with current approaches. We hypothesized platelet electrical resistance (PER) could be used for more comprehensive phenotyping of platelets. METHODS: Platelets were isolated from male and female healthy donors (age 39.6 ± 6.9) and septic patients (age 44.0 ± 13.5). PER 96-well plates were coated with various substrates, including fibrinogen and collagen. Platelets were added to the coated plates in the presence or absence of thrombin or convulxin. Platelet activation and spreading was monitored by measuring changes in electrical impedance. RESULTS: Platelets adhesion to fibrinogen and collagen increased impedance. In addition, impedance increased in response to thrombin or convulxin. No changes in impedance were observed in the absence of platelets or when wells were uncoated, indicating changes in impedance were directly due to platelet adhesion and activation. Inhibiting integrin αIIbβ3 decreased impedance when fibrinogen was used as a substrate, consistent with platelet-dependent effects. Platelets from septic patients caused increased impedance compared to healthy donors, demonstrating this assay can be used to assess platelet hyperreactivity. CONCLUSION: PER can be applied as a high throughput tool to measure platelet reactivity in health and disease, where platelet activation is increased.
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